YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells
文献类型：期刊论文
| 作者 | Zhao YuRong5; Qu Wei5; Liu WenYuan4; Hong Hao3; Feng Feng5; Chen Han2; Xie Ning1 |
| 刊名 | CHINESE JOURNAL OF NATURAL MEDICINES
 |
| 出版日期 | 2015 |
| 卷号 | 13期号:6页码:438 |
| 关键词 | OXIDATIVE STRESS INDUCED CYTOTOXICITY ALZHEIMERS-DISEASE FERULIC ACID MITOCHONDRIA DEATH SYMPTOMS DEMENTIA DAMAGE BETA YGS40 Yi-Gan San Hydrogen peroxide Antioxidant effects Apoptosis |
| ISSN号 | 2095-6975 |
| 英文摘要 | In our previous study, we have elucidated the chemical profile of YGS40, a fraction of Yi-Gan San (YGS), used for the treatment of Alzheimer's disease (AD). Oxidative stress-induced apoptosis is implicated in neurodegenerative disorders such as AD. The aim of the present study was to explore the protective effects of YGS40 against hydrogen peroxide (H2O2)-induced apoptosis in PC 12 cells and the underlying mechanisms. PC12 cells were exposed to 100 mu mol.L-1 of H2O2 for 12 h with or without YGS40 pretreatment. Cytotoxicity was determined by MTT (3, (4, 5-dimethylthiazole-2-yl) 2, 5-diphenyl-tetrazolium bromide) and lactate dehydrogenase (LDH) release assays; apoptosis was detected by Annexin V/propidium iodide coupled staining and by determining caspase-3 activity and Bax and Bcl-2 protein levels. Mitochondrial membrane potential (MMP) was assessed by the retention of rhodamine123; and the activities of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured using commercially available enzymatic kits. Pretreatment with YGS40 significantly prevented H2O2-induced cytotoxicity and protected the cells against H2O2-triggered apoptosis characterized by externalization of membrane phosphatidylserine and caspase-3 activation and the increased ratio of Bax/Bcl-2 in PC12 cells. Further studies showed that YGS40 suppressed H2O2-induced MMP loss, increased SOD activity, and decreased MDA level. These findings suggest that YGS40 may be beneficial for the prevention and treatment of oxidative stress-mediated disorders. |
| 资助项目 | [National Natural Science Foundation of China] |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/294685]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.Jiangxi Qingfeng Pharmaceut Corp, Ganzhou 341000, Peoples R China 2.陕西省地震局 3.安徽肥东花生原种场 4.中国科学院南京地理与湖泊研究所 5.中国科学院上海药物研究所 |
| 推荐引用方式 GB/T 7714 | Zhao YuRong,Qu Wei,Liu WenYuan,et al. YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2015,13(6):438. |
| APA | Zhao YuRong.,Qu Wei.,Liu WenYuan.,Hong Hao.,Feng Feng.,...&Xie Ning.(2015).YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells.CHINESE JOURNAL OF NATURAL MEDICINES,13(6),438. |
| MLA | Zhao YuRong,et al."YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells".CHINESE JOURNAL OF NATURAL MEDICINES 13.6(2015):438. |
入库方式： OAI收割
来源：上海药物研究所
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