The Global Boundary Stratotype Section and Point (GSSP) of the Jurassic-Cretaceous boundary (JKB) is the last among the GSSPs in the Phanerozoic to be chosen. The Berriasian Working Group of the International Subcommission on Cretaceous Stratigraphy decided in 2016 to use the base of the Calpionella alpina Subzone as the primary marker for the formal definition of the JKB. This definition is satisfactorily applicable for near continent, shallow offshore deposits within the Western Tethys, North Atlantic and central-south America. Unfortunately, the primary marker taxon cannot be found in the Pacific and circum-Pacific regions since the distribution of calpionellids is limited to the Western Tethys and neighboring regions. Radiolarians are good candidates for defining the JKB, because they are widespread and can be found both in shallow and deep pelagic sedimentary facies worldwide. We carried out detailed field observations and careful sample collections in a 5-m thick interval around the JKB in the Bosso Valley section (Northern Apennines), which is one of potential candidates for GSSP of the JKB. The Maiolica Formation, which includes the JKB, is characterized by white to light gray, well-bedded micritic limestones with abundant light gray to black chert layers and nodules. In this study, after selection of samples acid-etched in the field, we observed the etched surface with a scanning electron microscope in the laboratory. Our detailed observations revealed that both micritic limestone and chert generally contain abundant radiolarian tests. Most of them in limestones are replaced by calcite, so that it is impossible to isolate radiolarian tests from rock samples with acid treatment. On the other hand, radiolarian tests within chert layers or nodules are too recrystallized to preserve their original surface ornamentation. The best preserved radiolarian tests are included in micritic limestone near chert layer or nodules. Careful and systematic sampling enabled us to get enough material to establish phylogenetic analysis on radiolarians. (C) 2020 Elsevier Ltd. All rights reserved.