Novel keratinolytic enzymes, discovered from a talented and efficient bacterial keratin degrader
文献类型：期刊论文
| 作者 | Huang, Yuhong3,6; Lezyk, Mateusz2,6; Herbst, Florian-Alexander5; Busk, Peter Kamp4,6; Lange, Lene1,6 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2020-06-22 |
| 卷号 | 10期号:1页码:11 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-020-66792-2 |
| 英文摘要 | Huge quantities of keratinaceous waste are a substantial and almost totally unexploited protein resource which could be upgraded for use as high value-added products by efficient keratinolytic enzymes. In this study, we found that Bacillus sp. 8A6 can efficiently degrade chicken feather after 24h growth. According to phylogenetic analysis, the strain (formerly identified as Bacillus pumilus 8A6) belongs to the B. pumilus species clade but it is more closely related to B. safensis. Hotpep predicted 233 putative proteases from Bacillus sp. 8A6 genome. Proteomic analysis of culture broths from Bacillus sp. 8A6 cultured on chicken feathers or on a mixture of bristles and hooves showed high abundance of proteins with functions related to peptidase activity. Five proteases (one from family M12, one from family S01A, two from family S08A and one from family T3) and four oligopeptide and dipeptide binding proteins were highly expressed when Bacillus sp. 8A6 was grown in keratin media compared to LB medium. This study is the first to report that bacterial proteases in families M12, S01A and T3 are involved in keratin degradation together with proteases from family S08. |
| WOS关键词 | M12 METALLOPROTEASE ; MICROBIAL GENOMES ; BACILLUS ; DEGRADATION ; DECOMPOSITION ; PURIFICATION ; HYDROLYSIS ; MYROILYSIN ; PREDICTION ; PROTEASES |
| 资助项目 | Danish Innovation Fund[1308-00015B] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000543966000012 |
| 出版者 | NATURE PUBLISHING GROUP |
| 资助机构 | Danish Innovation Fund |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/41300]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Lange, Lene |
| 作者单位 | 1.Bioecon Res & Advisory, Karensgade 5, DK-2500 Valby, Denmark 2.Poznan Univ Tech, Fac Environm Engn & Energy, Water Supply & Bioecon Div, Berdychowo 4, PL-60965 Poznan, Poland 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Multiphase Complex Syst, Beijing Key Lab Ion Liquids Clean Proc,Key Lab Gr, Beijing 100190, Peoples R China 4.Roskilde Univ, Dept Sci & Environm, Univ Vej 1, DK-4000 Roskilde, Denmark 5.Aalborg Univ, Ctr Microbial Communities, Dept Chem & Biosci, Fredrik Bajers Vej 7H, DK-9220 Aalborg, Denmark 6.Tech Univ Denmark, Dept Biotechnol & Biomed, Bldg 224, DK-2800 Lyngby, Denmark |
| 推荐引用方式 GB/T 7714 | Huang, Yuhong,Lezyk, Mateusz,Herbst, Florian-Alexander,et al. Novel keratinolytic enzymes, discovered from a talented and efficient bacterial keratin degrader[J]. SCIENTIFIC REPORTS,2020,10(1):11. |
| APA | Huang, Yuhong,Lezyk, Mateusz,Herbst, Florian-Alexander,Busk, Peter Kamp,&Lange, Lene.(2020).Novel keratinolytic enzymes, discovered from a talented and efficient bacterial keratin degrader.SCIENTIFIC REPORTS,10(1),11. |
| MLA | Huang, Yuhong,et al."Novel keratinolytic enzymes, discovered from a talented and efficient bacterial keratin degrader".SCIENTIFIC REPORTS 10.1(2020):11. |
入库方式： OAI收割
来源：过程工程研究所
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