Characterization and stabilization in process development and product formulation for super large proteinaceous particles
文献类型：期刊论文
| 作者 | Yang, Yanli; Su, Zhiguo; Ma, Guanghui; Zhang, Songping |
| 刊名 | ENGINEERING IN LIFE SCIENCES
 |
| 出版日期 | 2020-07-19 |
| 页码 | 15 |
| 关键词 | characterization denaturation rational design super large proteinaceous particles |
| ISSN号 | 1618-0240 |
| DOI | 10.1002/elsc.202000033 |
| 英文摘要 | Super large proteinaceous particles (SLPPs) such as virus, virus like particles, and extracellular vesicles have successful and promising applications in vaccination, gene therapy, and cancer treatment. The unstable nature, the complex particulate structure and composition are challenges for their manufacturing and applications. Rational design of the processing should be built on the basis of fully understanding the characteristics of these bio-particles. This review highlights useful analytical techniques for characterization and stabilization of SLPPs in the process development and product formulations, including high performance size exclusion chromatography, multi-angle laser light scattering, asymmetrical flow field-flow fractionation, nanoparticle tracking analysis, CZE, differential scanning calorimetry, differential scanning fluorescence, isothermal titration calorimetry , and dual polarization interferometry. These advanced analytical techniques will be helpful in obtaining deep insight into the mechanism related to denaturation of SLPPs, and more importantly, in seeking solutions to preserve their biological functions against deactivation or denaturation. Combination of different physicochemical techniques, and correlation with in vitro or in vivo biological activity analyses, are considered to be the future trend of development in order to guarantee a high quality, safety, and efficacy of SLPPs. |
| WOS关键词 | VIRUS-LIKE PARTICLES ; B SURFACE-ANTIGEN ; DIFFERENTIAL SCANNING CALORIMETRY ; ISOTHERMAL TITRATION CALORIMETRY ; NANOPARTICLE TRACKING ANALYSIS ; ION-EXCHANGE CHROMATOGRAPHY ; FIELD-FLOW FRACTIONATION ; CAPILLARY-ELECTROPHORESIS ; HUMAN RHINOVIRUS ; INFLUENZA-VIRUS |
| 资助项目 | National Natural Science Foundation of China[21808226] ; National Natural Science Foundation of China[21821005] ; National Natural Science Foundation of China[31970872] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000549860900001 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/41431]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhang, Songping |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yanli,Su, Zhiguo,Ma, Guanghui,et al. Characterization and stabilization in process development and product formulation for super large proteinaceous particles[J]. ENGINEERING IN LIFE SCIENCES,2020:15. |
| APA | Yang, Yanli,Su, Zhiguo,Ma, Guanghui,&Zhang, Songping.(2020).Characterization and stabilization in process development and product formulation for super large proteinaceous particles.ENGINEERING IN LIFE SCIENCES,15. |
| MLA | Yang, Yanli,et al."Characterization and stabilization in process development and product formulation for super large proteinaceous particles".ENGINEERING IN LIFE SCIENCES (2020):15. |
入库方式： OAI收割
来源：过程工程研究所
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