From molasses to syrup: Engineering ultrafiltration membrane surface to improve invertase reusability
文献类型：期刊论文
| 作者 | Wang, Jingyu2,3; Luo, Jianquan2,3; Ren, Yuling3; Su, Ziran1; Guo, Shiwei3; Woodley, John M.1; Wan, Yinhua3 |
| 刊名 | JOURNAL OF MEMBRANE SCIENCE
 |
| 出版日期 | 2020-09-01 |
| 卷号 | 610页码:10 |
| ISSN号 | 0376-7388 |
| 关键词 | Ultrafiltration Antifouling Surface modification Polydopamine Enzyme reuse |
| DOI | 10.1016/j.memsci.2020.118287 |
| 英文摘要 | A by-product from membrane-mediated molasses refining process mainly contains sucrose, glucose, fructose and KCl, which is an ideal raw material to produce high fructose syrup (HFS) by invertase. Ultrafiltration (UF) membrane can achieve invertase reuse while protein adsorption on the membrane not only decreases the membrane permeability, but also induces enzyme activity loss. In this work, the effect of glucose/fructose and KCl on the catalytic behavior of invertase was investigated. Then the reusability of invertase for sucrose hy-drolysis was evaluated by different UF membranes, and mussel-inspired surface modification was employed to further improve the invertase reusability. The results showed that the presence of KCl in the raw materials greatly decreased the reaction rate, and thus desalination and concentration of the raw materials should be conducted before enzymatic hydrolysis to improve the enzymatic hydrolysis efficiency, save energy and reduce reactor volume. Moreover, it was found that the sucrose conversion decline with enzyme reuse by UF was caused by enzyme adsorption in/on the membrane and/or its penetration through the membrane leading to overall enzyme loss in the retentate. A polyether sulfone (PES) membrane with molecular weight cut-off (MWCO) of 10 kDa (UP010) was selected to recycle invertase because it enabled complete invertase retention and relatively high permeate flux. When L-cysteine (Cys) was grafted on the polydopamine-coated UP010 membrane, it could reduce invertase adsorption on the membrane, and thus keep high sucrose conversion (93-95%) in four enzyme reuse cycles because the grafted Cys greatly increased the negative charge and hydrophilicity of the membrane surface. This work not only offered an alternative for HFS production by membrane technology but also developed a novel strategy to construct antifouling surface to retard enzyme adsorption. |
| WOS关键词 | ENZYME IMMOBILIZATION ; ANTIFOULING PROPERTY ; MOLECULAR-WEIGHT ; CANE MOLASSES ; FILTRATION ; REMOVAL ; HYDROLYSIS ; DOPAMINE ; COATINGS ; SUCROSE |
| 资助项目 | Guangxi Science and Technology Major Project[AA17204034] |
| WOS研究方向 | Engineering ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000555548500006 |
| 资助机构 | Guangxi Science and Technology Major Project |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/41630]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Luo, Jianquan; Wan, Yinhua |
| 作者单位 | 1.Tech Univ Denmark, Dept Chem & Biochem Engn, DK-2800 Lyngby, Denmark 2.Univ Chinese Acad Sci, Sino Danish Coll, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jingyu,Luo, Jianquan,Ren, Yuling,et al. From molasses to syrup: Engineering ultrafiltration membrane surface to improve invertase reusability[J]. JOURNAL OF MEMBRANE SCIENCE,2020,610:10. |
| APA | Wang, Jingyu.,Luo, Jianquan.,Ren, Yuling.,Su, Ziran.,Guo, Shiwei.,...&Wan, Yinhua.(2020).From molasses to syrup: Engineering ultrafiltration membrane surface to improve invertase reusability.JOURNAL OF MEMBRANE SCIENCE,610,10. |
| MLA | Wang, Jingyu,et al."From molasses to syrup: Engineering ultrafiltration membrane surface to improve invertase reusability".JOURNAL OF MEMBRANE SCIENCE 610(2020):10. |
入库方式： OAI收割
来源：过程工程研究所
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