Molecular and functional characterization of the indoleamine 2,3-dioxygenase in grass carp (Ctenopharyngodon idella)
文献类型：期刊论文
| 作者 | Cui, Zheng-Wei1,4; Zhang, Xiang-Yang1,4; Zhang, Xu-Jie2; Wu, Nan1; Lu, Long-Feng1; Li, Shun1; Chen, Dan-Dan1; Zhang, Yong-An1,2,3 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2019-06-01 |
| 卷号 | 89页码:301-308 |
| 关键词 | Indoleamine 2,3-dioxygenase (IDO) Tryptophan TGF-beta 1 Immunoregulation Grass carp |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2019.04.005 |
| 英文摘要 | Indoleamine 2,3-dioxygenase (IDO) is a kind of dioxygenase that can catalyze the degradation of levo-tryptophan (L-Trp) and plays key roles in immune tolerance. In this study, the DO gene was cloned and functionally characterized from grass carp (gcIDO). The results showed that gcIDO overexpressed in GCO cells could catalyze the degradation of L-Trp through the L-Trp kynurenine pathway, and this activity could be promoted by delta-aminolevulinic acid (ALA) while inhibited by levo-1-methyl tryptophan (L-1MT). Moreover, gcIDO was constitutively expressed in various tissues, and its expression could be significantly up-regulated by LPS and Poly (I:C) in peripheral blood leukocytes (Pins). Furthermore, recombinant TGF-beta 1 of grass carp could up-regulate the expression of IDO, TGF-beta 1, CD25, and Foxp3 in PBLs, indicating that the TGF-beta 1/IDO pathway is present in fish. In the soybean meal induced enteritis (SBMIE) model, the expression of gcIDO in the intestine was upregulated significantly, demonstrating that gcIDO may play an immunoregulatory role in SBMIE. Taken together, these data suggest that the IDO plays multiple roles in the immunity of fish. |
| 资助项目 | National Natural Science Foundation of China[31472307] ; National Basic Research Program of China[2014CB138601] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000469897700034 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7423]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yong-An |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Zheng-Wei,Zhang, Xiang-Yang,Zhang, Xu-Jie,et al. Molecular and functional characterization of the indoleamine 2,3-dioxygenase in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2019,89:301-308. |
| APA | Cui, Zheng-Wei.,Zhang, Xiang-Yang.,Zhang, Xu-Jie.,Wu, Nan.,Lu, Long-Feng.,...&Zhang, Yong-An.(2019).Molecular and functional characterization of the indoleamine 2,3-dioxygenase in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,89,301-308. |
| MLA | Cui, Zheng-Wei,et al."Molecular and functional characterization of the indoleamine 2,3-dioxygenase in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 89(2019):301-308. |
入库方式： OAI收割
来源：武汉植物园
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