An efficient callus-based in vitro regeneration protocol for Warburgia Ugandensis Sprague, an important medicinal plant in Africa
文献类型：期刊论文
| 作者 | Zhang, Lingling1; Lu, Shiyou1,2; Yang, Baiming3; Parsons, Eugene P.4 |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
 |
| 出版日期 | 2019-02-01 |
| 卷号 | 55期号:1页码:3-8 |
| 关键词 | Warburgia ugandensis Callus-based regeneration Root induction percentage Plant growth regulators |
| ISSN号 | 1054-5476 |
| DOI | 10.1007/s11627-019-09961-x |
| 英文摘要 | Warburgia ugandensis Sprague is a woody species in the family Canellaceae and an important source of medicines in Africa. Natural propagation of W. ugandensis is problematic due to its recalcitrant seeds and lack of an efficient in vitro regeneration system for this species. This study describes an efficient regeneration protocol. Petiole bases and shoot tips were used as explants. Callus tissue developed when the explants were cultured on Murashige and Skoog medium containing 30gL(-1) sucrose and 7gL(-1) agar (MS30 medium), supplemented with 1.0mgL(-1) indole-3-butyric acid (IBA), 1.6mgL(-1)6-benzylaminopurine (BA), and 0.1mgL(-1) thidiazuron (TDZ). Adventitious buds were efficiently induced from the callus when the MS30 medium was supplemented with 0.8mgL(-1) BA and 0.2mgL(-1) IBA. Root induction occurred within 7-10d on half-strength MS30 medium supplemented with 0.8-1.0mgL(-1) 1-napthalene acetic acid (NAA), 0.2mgL(-1) IBA, and 0.03% (w/v) activated charcoal (AC). Roots were followed by root elongation on the same medium but lacking NAA and IBA. Approximately 50% of the plantlets cultured produced roots, while more than 80% of the plantlets survived and successfully grew to maturity. |
| 资助项目 | Wuhan Botanical Garden, Chinese Academy of Sciences[Y455442c03] |
| WOS研究方向 | Plant Sciences ; Cell Biology ; Developmental Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000460134100001 |
| 出版者 | SPRINGER |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7442]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Lingling |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 3.Changchun Guoxin Modern Agr Sci & Technol Dev Co, Changchun 130061, Jilin, Peoples R China 4.Prairie State Coll, Chicago Hts, IL 60411 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Lingling,Lu, Shiyou,Yang, Baiming,et al. An efficient callus-based in vitro regeneration protocol for Warburgia Ugandensis Sprague, an important medicinal plant in Africa[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2019,55(1):3-8. |
| APA | Zhang, Lingling,Lu, Shiyou,Yang, Baiming,&Parsons, Eugene P..(2019).An efficient callus-based in vitro regeneration protocol for Warburgia Ugandensis Sprague, an important medicinal plant in Africa.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,55(1),3-8. |
| MLA | Zhang, Lingling,et al."An efficient callus-based in vitro regeneration protocol for Warburgia Ugandensis Sprague, an important medicinal plant in Africa".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 55.1(2019):3-8. |
入库方式： OAI收割
来源：武汉植物园
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