Comparative study of the immunoprotective effect of two grass carp-sourced Bacillus subtilis spore-based vaccines against grass carp reovirus
文献类型：期刊论文
| 作者 | Chen, Dan-Dan3; Yao, Yuan-Yuan2,3; Cui, Zheng-Wei2,3; Zhang, Xiang-Yang2,3; Guo, Xia4; Zhou, Yuan-Yuan2,3; Zhang, Yong-An1,3 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2019-04-15 |
| 卷号 | 504页码:88-95 |
| 关键词 | Grass carp reovirus Bacillus subtilis spore Oral vaccine Immunoprotection Immune response |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2019.01.055 |
| 英文摘要 | Grass carp reovirus II (GCRV II) causes severe haemorrhagic disease with high mortality in grass carp, Cyenopharyngodon idellus. Vaccination is a very effective method in conferring protection against GCRV. In the current study, we constructed two grass carp-sourced Bacillus subtilis spore-based vaccines (GC5-VP4 and GC5NS38) and investigated their potential as oral candidate vaccines against GCRV II. Our results showed that both VP4 and NS38 proteins of GCRV II were efficiently displayed on the spore surface of B. subtilis GC5, and the two recombinant spores stayed in the hindguts of grass carp for antigen presentation. After oral administration, compared with the control groups, both recombinant vaccines GC5-VP4 and GC5-NS38 increased the survival rate of grass carp against GCRV II, with relative percent survival rates of 30% and 36.4%, respectively. Although they had a similar immunoprotective effect, the mechanisms were very different. GC5-VP4 mainly elicited specific antibody responses, both systemic and mucosal, whereas GC5-NS38 mainly elicited an inflammatory response and cellular immunity via expression of major histocompatibility complex molecules. Furthermore, this study demonstrated that the recombinant spores triggered high levels of mucosal immunity and would be promising oral candidate vaccines against GCRV II in grass carp. Combined with previous research, we inferred that vaccine efficacy was not only related to antigen, but also vaccination strategies. |
| 资助项目 | National Natural Science Foundation of China[31725026] ; Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province of China[2018CFA011] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000459962400011 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7513]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yong-An |
| 作者单位 | 1.Huazhong Agr Univ, Coll Fisheries, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China 4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Dan-Dan,Yao, Yuan-Yuan,Cui, Zheng-Wei,et al. Comparative study of the immunoprotective effect of two grass carp-sourced Bacillus subtilis spore-based vaccines against grass carp reovirus[J]. AQUACULTURE,2019,504:88-95. |
| APA | Chen, Dan-Dan.,Yao, Yuan-Yuan.,Cui, Zheng-Wei.,Zhang, Xiang-Yang.,Guo, Xia.,...&Zhang, Yong-An.(2019).Comparative study of the immunoprotective effect of two grass carp-sourced Bacillus subtilis spore-based vaccines against grass carp reovirus.AQUACULTURE,504,88-95. |
| MLA | Chen, Dan-Dan,et al."Comparative study of the immunoprotective effect of two grass carp-sourced Bacillus subtilis spore-based vaccines against grass carp reovirus".AQUACULTURE 504(2019):88-95. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。