HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex
文献类型：期刊论文
| 作者 | Bejarano, David Alejandro2; Peng, Ke2,4; Laketa, Vibor2,3; Boerner, Kathleen2,3; Jost, K. Laurence2,3; Lucic, Bojana1,3; Glass, Baerbel2; Lusic, Marina1,3; Mueller, Barbara2; Kraeusslich, Hans-Georg2 |
| 刊名 | ELIFE
 |
| 出版日期 | 2019-01-23 |
| 卷号 | 8页码:31 |
| ISSN号 | 2050-084X |
| DOI | 10.7554/eLife.41800 |
| 英文摘要 | Nuclear entry of HIV-1 replication complexes through intact nuclear pore complexes is critical for successful infection. The host protein cleavage-and-polyadenylation-specificity-factor-6 (CPSF6) has been implicated in different stages of early HIV-1 replication. Applying quantitative microscopy of HIV-1 reverse-transcription and pre-integration-complexes (RTC/PIC), we show that CPSF6 is strongly recruited to nuclear replication complexes but absent from cytoplasmic RTC/PIC in primary human macrophages. Depletion of CPSF6 or lack of CPSF6 binding led to accumulation of HIV-1 subviral complexes at the nuclear envelope of macrophages and reduced infectivity. Two-color stimulated-emission-depletion microscopy indicated that under these circumstances HIV-1 complexes are retained inside the nuclear pore and undergo CA-multimer dependent CPSF6 clustering adjacent to the nuclear basket. We propose that nuclear entry of HIV-1 subviral complexes in macrophages is mediated by consecutive binding of Nup153 and CPSF6 to the hexameric CA lattice. |
| 资助项目 | Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)[240245660 - SFB 1129] ; TTU HIV in the DZIF ; HBIGS graduate school, Heidelberg ; [SPP1923] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000460405100001 |
| 出版者 | ELIFE SCIENCES PUBLICATIONS LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7570]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Peng, Ke; Kraeusslich, Hans-Georg |
| 作者单位 | 1.Heidelberg Univ, Dept Infect Dis, Integrat Virol, Heidelberg, Germany 2.Heidelberg Univ, Dept Infect Dis Virol, Heidelberg, Germany 3.German Ctr Infect Res, Heidelberg, Germany 4.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bejarano, David Alejandro,Peng, Ke,Laketa, Vibor,et al. HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex[J]. ELIFE,2019,8:31. |
| APA | Bejarano, David Alejandro.,Peng, Ke.,Laketa, Vibor.,Boerner, Kathleen.,Jost, K. Laurence.,...&Kraeusslich, Hans-Georg.(2019).HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex.ELIFE,8,31. |
| MLA | Bejarano, David Alejandro,et al."HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex".ELIFE 8(2019):31. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。