Detection of West Nile virus lineage 1 sequences in blood donors, Punjab Province, Pakistan
文献类型：期刊论文
| 作者 | Zohaib, Ali2,3; Niazi, Saifullah Khan4; Saqib, Muhammad5; Sajid, Muhammad Sohail5; Khan, Iahtasham6; Sial, Awais-ur-Rahman7; Athar, Muhammad Ammar8; Taj, Zeeshan9; Abbas, Ghazanfar5; Rathore, Muhammad Ali1 |
| 刊名 | INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
 |
| 出版日期 | 2019-04-01 |
| 卷号 | 81页码:137-139 |
| ISSN号 | 1201-9712 |
| 关键词 | West Nile virus Mosquitoes Human Pakistan |
| DOI | 10.1016/j.ijid.2019.01.020 |
| 英文摘要 | Objectives: This study was performed to determine the presence of West Nile virus (WNV) in mosquito specimens and human blood donors in Pakistan. Methods: A total of 4150 mosquito specimens were collected using CO2-baited traps from five selected districts of Punjab Province, Pakistan. The mosquitoes were taxonomically identified using standard morphological keys, resulting in 166 pools. In addition, 1070 serum samples were collected from human blood donors. RNA was extracted from mosquito and human samples and screened for WNV using a reverse transcriptase PCR (RT-PCR). Results: None of the mosquito pools tested positive for WNV, whereas three samples from asymptomatic humans tested positive. To determine the WNV strains, partial sequences were compared against a global representation of 23 WNV sequences. The study strains were determined to come from WNV lineage 1. Conclusions: This study is novel in reporting the circulation of lineage 1 WNV in Pakistan. Given its ability to transmit from human to human via blood transfusion, this highlights the urgent need for nationwide surveillance to assess the distribution and impact of WNV in Pakistan. Determining the source of human infection will require more extensive mosquito sampling. (C) 2019 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. |
| 资助项目 | International Cooperation on Key Technologies of Biosafety along the China-Pakistan Economic Corridor[153B42KYSB20170004] ; External Cooperation Program of CAS[153211KYSB20160001] ; Higher Education Commission, Pakistan[SRGP-1055] |
| WOS研究方向 | Infectious Diseases |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000463889700027 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7685]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Saqib, Muhammad |
| 作者单位 | 1.AFIT, Rawalpindi, Pakistan 2.Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.AFIP, Rawalpindi, Pakistan 5.Univ Agr Faisalabad, Fac Vet Med, Faisalabad, Pakistan 6.Univ Vet & Anim Sci, Dept Clin Sci, Sect Epidemiol & Publ Hlth, Lahore Subcampus, Jhang, Pakistan 7.PMAS Aird Agr Univ, Fac Vet & Anim Sci, Rawalpindi, Pakistan 8.Henan Univ, Coll Pharm, Lab Canc Biomarkers & Liquid Biopsy, Kaifeng, Henan, Peoples R China 9.Govt Coll Univ Faisalabad, Dept Microbiol, Faisalabad, Punjab, Pakistan 10.Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zohaib, Ali,Niazi, Saifullah Khan,Saqib, Muhammad,et al. Detection of West Nile virus lineage 1 sequences in blood donors, Punjab Province, Pakistan[J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES,2019,81:137-139. |
| APA | Zohaib, Ali.,Niazi, Saifullah Khan.,Saqib, Muhammad.,Sajid, Muhammad Sohail.,Khan, Iahtasham.,...&Shi, Zheng-Li.(2019).Detection of West Nile virus lineage 1 sequences in blood donors, Punjab Province, Pakistan.INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES,81,137-139. |
| MLA | Zohaib, Ali,et al."Detection of West Nile virus lineage 1 sequences in blood donors, Punjab Province, Pakistan".INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES 81(2019):137-139. |
入库方式： OAI收割
来源：武汉植物园
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