Integrated mRNA and miRNA expression profile analyses reveal the potential roles of sex-biased miRNA-mRNA pairs in gonad tissues of the Chinese concave-eared torrent frog (Odorrana tormota)
文献类型：期刊论文
| 作者 | Shu, Yilin3,4; Zhang, Huijuan3,4; Cai, Qijia1,5; Tang, Dong3,4; Wang, Gang3,4; Liu, Ting3,4; Lv, Bihua2; Wu, Hailong3,4 |
| 刊名 | JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
 |
| 出版日期 | 2019-05-01 |
| 卷号 | 332期号:3-4页码:69-80 |
| 关键词 | gonad miRNAs mRNAs Odorrana tormota reproduction and sex differentiation |
| ISSN号 | 1552-5007 |
| DOI | 10.1002/jez.b.22851 |
| 英文摘要 | The Chinese concave-eared torrent frog (Odorrana tormota) is typically sexually dimorphic. Females are significantly less common than males in the wild. Until now, the molecular mechanisms of reproduction and sex differentiation of frogs remain unclear. Here, we integrated mRNA and microRNA (miRNA) expression profiles to reveal the molecular mechanisms of reproduction and sex differentiation in O. tormota. We identified 234 differentially expressed miRNAs (DEMs) and 18,551 differentially expressed transcripts. Of these, 12,053 mRNAs and 64 miRNAs were upregulated in testes, and 6,498 mRNAs and 170 miRNAs were upregulated in ovaries. Integrated analysis of the miRNA and mRNA expression profiles predicted 75,602 potential miRNA-mRNA interaction sites, with 42,065 negative miRNA-mRNA interactions. We found 36 differentially expressed genes (DEGs) related to reproduction and sex differentiation, of which 15 DEGs formed 92 negative miRNA-mRNA interactions with 34 known DEMs. Thus, miRNAs may play other important roles in O. tormota. Furthermore, Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses showed reproductive-related processes, such as the gonadotropinreleasing hormone signaling pathway and ovarian steroidogenesis. Based on functional annotation and the literature, the retinoic acid signaling pathway, the SOX9-AMH pathway, and the process of spermatogenesis may be involved in the molecular mechanisms of reproduction and sex differentiation in O. tormota, and may be regulated by miRNAs. The miRNA-mRNA pairs described may provide further understanding of the regulatory mechanisms associated with reproduction and sex differentiation, and the molecular mechanism of reproduction in O. tormota. |
| 资助项目 | National Natural Science Foundation of China[31370537] ; Doctoral Fund of Ministry of Education of China[20133424110006] |
| WOS研究方向 | Evolutionary Biology ; Developmental Biology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000468966100002 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7779]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Lv, Bihua; Wu, Hailong |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Wuhan Univ, Zhongnan Hosp, Dept Pharm, Wuhan, Hubei, Peoples R China 3.Key Lab Conservat & Utilizat Important Biol Resou, Wuhu, Peoples R China 4.Anhui Normal Univ, Coll Life Sci, Wuhu, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shu, Yilin,Zhang, Huijuan,Cai, Qijia,et al. Integrated mRNA and miRNA expression profile analyses reveal the potential roles of sex-biased miRNA-mRNA pairs in gonad tissues of the Chinese concave-eared torrent frog (Odorrana tormota)[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,2019,332(3-4):69-80. |
| APA | Shu, Yilin.,Zhang, Huijuan.,Cai, Qijia.,Tang, Dong.,Wang, Gang.,...&Wu, Hailong.(2019).Integrated mRNA and miRNA expression profile analyses reveal the potential roles of sex-biased miRNA-mRNA pairs in gonad tissues of the Chinese concave-eared torrent frog (Odorrana tormota).JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,332(3-4),69-80. |
| MLA | Shu, Yilin,et al."Integrated mRNA and miRNA expression profile analyses reveal the potential roles of sex-biased miRNA-mRNA pairs in gonad tissues of the Chinese concave-eared torrent frog (Odorrana tormota)".JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION 332.3-4(2019):69-80. |
入库方式： OAI收割
来源：武汉植物园
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