Synaptosomal-associated protein 29 is required for the autophagic degradation of hepatitis B virus
文献类型：期刊论文
| 作者 | Lin, Yong3; Wu, Chunchen5; Wang, Xueyu3; Liu, Shi1,3; Kemper, Thekla3; Li, Fahong2,3; Squire, Anthony4; Zhu, Ying1; Zhang, Jiming2; Chen, Xinwen5 |
| 刊名 | FASEB JOURNAL
 |
| 出版日期 | 2019-05-01 |
| 卷号 | 33期号:5页码:6023-6034 |
| ISSN号 | 0892-6638 |
| 关键词 | HBV SNAP29 VAMP8 autophagy |
| DOI | 10.1096/fj.201801995RR |
| 英文摘要 | Hepatitis B virus (HBV) replication and envelopment is dependent on cellular autophagy. Previously, we have provided evidence for the extensive lysosomal degradation of HBV virions and the hepatitis B surface antigen (HBsAg), which is likely controlled by autophagosome-lysosome fusion. Synaptosomal-associated protein 29 (SNAP29) has been identified as a protein specifically mediating autophagosome-lysosome fusion. Thus, in the present study, we addressed the hypothesis that SNAP29 is required for the autophagic degradation of HBV virions and HBsAg. We found that silencing SNAP29 significantly increased the number of autophagosomes and concomitantly promoted HBV replication and HBsAg production. Conversely, SNAP29 overexpression decreased HBV production. Consistent with this, SNAP29 modulated HBV production by interacting with vesicle-associated membrane protein 8 (VAMP8) and synergistically regulated HBV replication with Rab7 complexes. Moreover, the production and release of the small HBsAg is strongly regulated by SNAP29 expression, suggesting that its export occurs partly through the autophagic pathway. Our findings provide new evidence, strongly suggesting that autophagic degradation critically determines the production of HBV virions and HBsAg and that this is controlled by the SNAP29-VAMP8 interaction.Lin, Y., Wu, C., Wang, X., Liu, S., Kemper, T., Li, F., Squire, A., Zhu, Y., Zhang, J., Chen, X., Lu, M. Synaptosomal-associated protein 29 is required for the autophagic degradation of hepatitis B virus. |
| 资助项目 | Deutsche Forschungsgemeinschaft[RTG1949/2] ; Deutsche Forschungsgemeinschaft[Transregio TRR60] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| 语种 | 英语 |
| 出版者 | FEDERATION AMER SOC EXP BIOL |
| WOS记录号 | WOS:000466932600019 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7812]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Lu, Mengji |
| 作者单位 | 1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan, Hubei, Peoples R China 2.Fudan Univ, Huashan Hosp, Dept Infect Dis, Shanghai, Peoples R China 3.Univ Duisburg Essen, Univ Hosp Essen, Inst Virol, Essen, Germany 4.Univ Duisburg Essen, Univ Hosp Essen, Inst Expt Immunol & Imaging, Essen, Germany 5.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Yong,Wu, Chunchen,Wang, Xueyu,et al. Synaptosomal-associated protein 29 is required for the autophagic degradation of hepatitis B virus[J]. FASEB JOURNAL,2019,33(5):6023-6034. |
| APA | Lin, Yong.,Wu, Chunchen.,Wang, Xueyu.,Liu, Shi.,Kemper, Thekla.,...&Lu, Mengji.(2019).Synaptosomal-associated protein 29 is required for the autophagic degradation of hepatitis B virus.FASEB JOURNAL,33(5),6023-6034. |
| MLA | Lin, Yong,et al."Synaptosomal-associated protein 29 is required for the autophagic degradation of hepatitis B virus".FASEB JOURNAL 33.5(2019):6023-6034. |
入库方式： OAI收割
来源：武汉植物园
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