Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level
文献类型：期刊论文
| 作者 | Wei, Li3,4,5; Shen, Chen3,4,5; El Hajjami, Mohamed1; You, Wuxin1,4,5; Wang, Qintao3,4,5; Zhang, Peng3,4,5; Ji, Yuetong3,4,5; Hu, Hanhua3,6; Hu, Qiang3,6; Poetsch, Ansgar1,2,4,5 |
| 刊名 | METABOLIC ENGINEERING
 |
| 出版日期 | 2019-07-01 |
| 卷号 | 54页码:96-108 |
| 关键词 | Carbon fixation Acidity tolerance Carbonic anhydrase Industrial oleaginous microalgae Nannochloropsis |
| ISSN号 | 1096-7176 |
| DOI | 10.1016/j.ymben.2019.03.004 |
| 英文摘要 | Improving acid tolerance is pivotal to the development of microalgal feedstock for converting flue gas to biomass or oils. In the industrial oleaginous microalga Nannochloropsis oceanica, transcript knockdown of a cytosolic carbonic anhydrase (CA2), which is a key Carbon Concentrating Mechanism (CCM) component induced under 100 ppm CO2 (very low carbon, or VLC), results in similar to 45%, similar to 30% and similar to 40% elevation of photosynthetic oxygen evolution rate, growth rate and biomass accumulation rate respectively under 5% CO2 (high carbon, or HC), as compared to the wild type. Such high-CO2-level activated biomass over-production is reproducible across photobioreactor types and cultivation scales. Transcriptomic, proteomic and physiological changes of the mutant under high CO2 (HC; 5% CO2) suggest a mechanism where the higher pH tolerance is coupled to reduced biophysical CCM, sustained pH hemostasis, stimulated energy intake and enhanced photosynthesis. Thus "inactivation of CCM" can generate hyper-CO2-assimilating and autonomously containable industrial microalgae for flue gas-based oil production. |
| 资助项目 | Natural Science Foundation of China[31425002] ; Natural Science Foundation of China[31741005] ; Biological Carbon Sequestration Program of Chinese Academy of Sciences[KSZD-EW-Z-017] ; Biological Carbon Sequestration Program of Chinese Academy of Sciences[ZDRW-ZS-2016-3] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000470680100008 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7933]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wei, Li; Poetsch, Ansgar; Xu, Jian |
| 作者单位 | 1.Ruhr Univ Bochum, Dept Plant Biochem, Bochum, Germany 2.Univ Plymouth, Sch Biomed & Healthcare Sci, Plymouth, Devon, England 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao, Shandong, Peoples R China 5.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 6.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Li,Shen, Chen,El Hajjami, Mohamed,et al. Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level[J]. METABOLIC ENGINEERING,2019,54:96-108. |
| APA | Wei, Li.,Shen, Chen.,El Hajjami, Mohamed.,You, Wuxin.,Wang, Qintao.,...&Xu, Jian.(2019).Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level.METABOLIC ENGINEERING,54,96-108. |
| MLA | Wei, Li,et al."Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level".METABOLIC ENGINEERING 54(2019):96-108. |
入库方式： OAI收割
来源：武汉植物园
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