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Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF-kappa B signalling pathway by binding to eEF1A1

文献类型：期刊论文

作者Wei, Sha5,6; Wang, Dianbing6; Li, Hua5,6; Bi, Lijun1,8; Deng, Jiaoyu9; Zhu, Guofeng1,8; Zhang, Jibin5; Li, Chuanyou4; Li, Min6; Fang, Yuan2
刊名CELLULAR MICROBIOLOGY
出版日期2019-08-09
页码15
ISSN号1462-5814
关键词cytokines fatty acylCoA synthetase Mycobacterium tuberculosis NF-kappa B signalling pathway
DOI10.1111/cmi.13090
英文摘要Mycobacterium tuberculosis (Mtb) manipulates multiple host defence pathways to survive and persist in host cells. Understanding Mtb-host cell interaction is crucial to develop an efficient means to control the disease. Here, we applied the Mtb proteome chip, through separately interacting with H37Ra and H37Rv stimulated macrophage lysates, screened 283 Mtb differential proteins. Through primary screening, we focused on fatty acylCoA synthetase FadD13. Mtb FadD13 is a potential drug target, but its role in infection remains unclear. Deletion of FadD13 in Mtb reduced the production of proinflammatory cytokines IL-1 beta, IL-18, and IL-6. Bimolecular fluorescence complementation and colocalization showed that the binding partner of FadD13 in macrophage was eEF1A1 (a translation elongation factor). Knockdown eEF1A1 expression in macrophage abrogated the promotion of proinflammatory cytokines induced by FadD13. In addition, Delta fadD13 mutant decreased the expression of the NF-kappa B signalling pathway related proteins p50 and p65, so did the eEF1A1 knockdown macrophage infected with H37Rv. Meanwhile, we found that deletion of FadD13 reduced Mtb survival in macrophages during Mtb infection, and purified FadD13 proteins induced broken of macrophage membrane. Taken together, FadD13 is crucial for Mtb proliferation in macrophages, and it plays a key role in the production of proinflammatory cytokines during Mtb infection.
资助项目National Science and Technology Major Project[2018ZX10731301-003-008] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB29050100] ; Chinese Academy of Sciences[KJZD-EW-TZ-L04]
WOS研究方向Cell Biology ; Microbiology
语种英语
出版者WILEY
WOS记录号WOS:000481276000001
源URL[http://202.127.146.157/handle/2RYDP1HH/7953]  
专题中国科学院武汉植物园
通讯作者Zhang, Xian-En
作者单位1.Chinese Acad Sci, Inst Biophys, State Key Lab Biomacromol, Beijing, Peoples R China
2.Hubei Univ, Coll Life Sci, Wuhan, Hubei, Peoples R China
3.Shanghai Jiao Tong Univ, Minist Educ, Key Lab Syst Biomed, Shanghai Ctr Syst Biomed, Shanghai, Peoples R China
4.Capital Med Univ, Beijing Chest Hosp, Beijing, Peoples R China
5.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agromicrobiol, Wuhan, Hubei, Peoples R China
6.Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
7.Univ Chinese Acad Sci, Beijing, Peoples R China
8.Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing, Peoples R China
9.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Wei, Sha,Wang, Dianbing,Li, Hua,et al. Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF-kappa B signalling pathway by binding to eEF1A1[J]. CELLULAR MICROBIOLOGY,2019:15.
APA Wei, Sha.,Wang, Dianbing.,Li, Hua.,Bi, Lijun.,Deng, Jiaoyu.,...&Zhang, Xian-En.(2019).Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF-kappa B signalling pathway by binding to eEF1A1.CELLULAR MICROBIOLOGY,15.
MLA Wei, Sha,et al."Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF-kappa B signalling pathway by binding to eEF1A1".CELLULAR MICROBIOLOGY (2019):15.

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