Free-base porphyrins as CEST MRI contrast agents with highly upfield shifted labile protons
文献类型：期刊论文
| 作者 | Zhang, Xiaoxiao3; Yuan, Yaping2,3; Li, Sha2,3; Zeng, Qingbin2,3; Guo, Qianni2,3; Liu, Na2,3; Yang, Minghui2,3; Yang, Yunhuang2,3; Liu, Maili2,3; McMahon, Michael T.1,4 |
| 刊名 | MAGNETIC RESONANCE IN MEDICINE
 |
| 出版日期 | 2019-08-01 |
| 卷号 | 82期号:2页码:577-585 |
| ISSN号 | 0740-3194 |
| 关键词 | CEST imaging contrast agents free-base porphyrin upfield-shifted protons molecular imaging |
| DOI | 10.1002/mrm.27753 |
| 英文摘要 | PurposeCEST has become a preeminent technology for the rapid detection and grading of tumors, securing its widespread use in both laboratory and clinical research. However, many existing CEST MRI agents exhibit a sensitivity limitation due to small chemical shifts between their exchangeable protons and water. We propose a new group of CEST MRI agents, free-base porphyrins and chlorin, with large exchangeable proton chemical shifts from water for enhanced detection. MethodsTo test these newly identified CEST agents, we acquired a series of Z-spectra at multiple pH values and saturation field strengths to determine their CEST properties. The data were analyzed using the quantifying exchange using saturation power method to quantify exchange rates. After identifying several promising candidates, a porphyrin solution was injected into tumor-bearing mice, and MR images were acquired to assess detection feasibility in vivo. ResultsBased on the Z-spectra, the inner nitrogen protons in free-base porphyrins and chlorin resonate from -8 to -13.5 ppm from water, far shifted from the majority of endogenous metabolites (0-4 ppm) and Nuclear Overhauser enhancements (-1 to -3.5 ppm) and far removed from the salicylates, imidazoles, and anthranillates (5-12 ppm). The exchange rates are sufficiently slow to intermediate (500-9000 s(-1)) to allow robust detection and were sensitive to substituents on the porphyrin ring. ConclusionThese results highlight the capabilities of free-base porphyrins and chlorin as highly upfield CEST MRI agents and provide a new scaffold that can be integrated into a variety of diagnostic or theranostic agents for biomedical applications. |
| 资助项目 | National Natural Science Foundation of China[81625011] ; National Natural Science Foundation of China[81227902] ; National Key R&D Program of China[2016YFC1304700] ; National Key R&D Program of China[2017YFA0505400] ; Key Research Program of Frontier Sciences, CAS[QYZDY-SSW-SLH018] ; National Program for Support of Eminent Professionals (National Program for Support of Top-notch Young Professionals) ; National Institutes of Health[P41EB024495] |
| WOS研究方向 | Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000471308300006 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8095]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhou, Xin |
| 作者单位 | 1.Johns Hopkins Univ, Sch Med, Russell H Morgan Dept Radiol, Baltimore, MD USA 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst,Wuhan Inst Phy, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Collaborat Innova, Wuhan, Hubei, Peoples R China 4.Kennedy Krieger Inst, FM Kirby Res Ctr Funct Brain Imaging, Baltimore, MD USA |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaoxiao,Yuan, Yaping,Li, Sha,et al. Free-base porphyrins as CEST MRI contrast agents with highly upfield shifted labile protons[J]. MAGNETIC RESONANCE IN MEDICINE,2019,82(2):577-585. |
| APA | Zhang, Xiaoxiao.,Yuan, Yaping.,Li, Sha.,Zeng, Qingbin.,Guo, Qianni.,...&Zhou, Xin.(2019).Free-base porphyrins as CEST MRI contrast agents with highly upfield shifted labile protons.MAGNETIC RESONANCE IN MEDICINE,82(2),577-585. |
| MLA | Zhang, Xiaoxiao,et al."Free-base porphyrins as CEST MRI contrast agents with highly upfield shifted labile protons".MAGNETIC RESONANCE IN MEDICINE 82.2(2019):577-585. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。