The Edwardsiella piscicida Type III Effector EseJ Suppresses Expression of Type 1 Fimbriae, Leading to Decreased Bacterial Adherence to Host Cells
文献类型：期刊论文
| 作者 | Zhang, Qian1,3,4; He, Tian Tian1,3,4; Li, Duan You1,3,4; Liu, Lu Yi1,4; Nie, Pin1,2,4,5; Xie, Hai Xia1,4 |
| 刊名 | INFECTION AND IMMUNITY
 |
| 出版日期 | 2019-07-01 |
| 卷号 | 87期号:7页码:13 |
| 关键词 | EseJ T3SS type 1 fimbriae adhesion |
| ISSN号 | 0019-9567 |
| DOI | 10.1128/IAI.00187-19 |
| 英文摘要 | The type III secretion system (T3SS) of Edwardsiella piscicida plays a crucial role in its pathogenesis. Our previous study indicated that the T3SS effector protein EseJ inhibits the bacterium's adhesion to epithelioma papillosum cyprini (EPC) cells, while the mechanism of the inhibition remains elusive. In this study, we revealed that EseJ negatively regulates the fimA gene, as demonstrated by comparative transcription analysis of Delta eseJ and wild-type (WT) strains. As well, the dramatically increased production of FimA was detected in the absence of EseJ compared to that by the WT strain. The adherence of the Delta eseJ strain decreased far below that of the WT strain in the absence of FimA, demonstrating that FimA plays a pivotal role in the hyperadhesion of the Delta eseJ strain. Adherence analysis with a strain with truncated eseJ demonstrated that the C-terminal region of EseJ (Gly1191 to Ile1359) is necessary to inhibit the transcription of the type 1 fimbrial operon. Binding between the EseJ fragment from amino acid residues 1191 to 1359 and the DNA fragment upstream of fimA was not detected, indicating that EseJ might indirectly regulate the type 1 fimbrial operon. Our study reveals that EseJ controls E. piscicida adherence to EPC cells by negatively regulating the type 1 fimbrial operon. |
| 资助项目 | National Natural Science Foundation of China (NSFC)[U1706205] ; China Agriculture Research System[CARS-46] |
| WOS研究方向 | Immunology ; Infectious Diseases |
| 语种 | 英语 |
| WOS记录号 | WOS:000472150800001 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8103]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Nie, Pin; Xie, Hai Xia |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan, Hubei, Peoples R China 2.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qian,He, Tian Tian,Li, Duan You,et al. The Edwardsiella piscicida Type III Effector EseJ Suppresses Expression of Type 1 Fimbriae, Leading to Decreased Bacterial Adherence to Host Cells[J]. INFECTION AND IMMUNITY,2019,87(7):13. |
| APA | Zhang, Qian,He, Tian Tian,Li, Duan You,Liu, Lu Yi,Nie, Pin,&Xie, Hai Xia.(2019).The Edwardsiella piscicida Type III Effector EseJ Suppresses Expression of Type 1 Fimbriae, Leading to Decreased Bacterial Adherence to Host Cells.INFECTION AND IMMUNITY,87(7),13. |
| MLA | Zhang, Qian,et al."The Edwardsiella piscicida Type III Effector EseJ Suppresses Expression of Type 1 Fimbriae, Leading to Decreased Bacterial Adherence to Host Cells".INFECTION AND IMMUNITY 87.7(2019):13. |
入库方式： OAI收割
来源：武汉植物园
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