Characterization of a Novel Tanay Virus Isolated From Anopheles sinensis Mosquitoes in Yunnan, China
文献类型：期刊论文
| 作者 | Zhao, Lu2,3; Mwaliko, Caroline2,3; Atoni, Evans2,3; Wang, Yujuan2,3; Zhang, Yunzhi1; Zhan, Jianbo4; Hu, Xiaomin2; Xia, Han2; Yuan, Zhiming2 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2019-08-22 |
| 卷号 | 10页码:11 |
| 关键词 | Tansy virus Negevirus insect specific viruses yunnan Anopheles sinensis |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2019.01963 |
| 英文摘要 | Globally, mosquitoes are known to be competent vectors to various arboviruses that cause serious and debilitating diseases to humans and animals. Conversely, mosquitoes harbor a wide array of insect specific viruses (ISVs) that are generally neglected. Extensive characterization of these ISVs is important in understanding their persistence infection effect on host behavior and arbovirus transmission. Herein, we report first time isolation of Tanay virus (TANAV) isolate YN15_103_01 in Anopheles sinensis mosquitoes from Yunnan Province, China. Phylogenetically, the isolate's nucleotide identity had more than 14.47% variance compared to previous TANAV isolates, and it clustered into an independent branch within the genus Sandewavirus in the newly proposed taxon Negevirus. TANAV growth and high titers was attained in Aag2 cells (10(7) PFU/mL) but with no CPE observed up to 7 days.p.i. compared to C6/36 cells that exhibited extensive CPE at 48 h.p.i. with titers of 10(7) PFU/mL. Contrarywise, the viral isolate did not replicate in vertebrate cell lines. Electron microscopy analyses showed that its final maturation process takes place in the cell cytoplasm. Notably, the predicted viral proteins were verified to be corresponding to the obtained SDS-PAGE protein bands. Our findings advance forth new and vital knowledge important in understanding insect specific viruses, especially TANAV. |
| 资助项目 | Ministry of Science and Technology of the People's Republic of China[2018ZX10101004] ; Ministry of Science and Technology of the People's Republic of China[2013FY113500] ; Chinese Academy of Sciences[153211KYSB20160001] ; Chinese Academy of Sciences[ZDRW-ZS-2016-4] ; Wuhan Institute of Virology, China[WIV-135-PY2] ; Health Commission of Hubei Province[WJ2019Q060] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000482190700001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8136]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Xia, Han; Yuan, Zhiming |
| 作者单位 | 1.Yunnan Inst Endem Dis Control & Prevent, Dali, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Hubei Prov Ctr Dis Control & Prevent, Div Viral Dis Detect, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Lu,Mwaliko, Caroline,Atoni, Evans,et al. Characterization of a Novel Tanay Virus Isolated From Anopheles sinensis Mosquitoes in Yunnan, China[J]. FRONTIERS IN MICROBIOLOGY,2019,10:11. |
| APA | Zhao, Lu.,Mwaliko, Caroline.,Atoni, Evans.,Wang, Yujuan.,Zhang, Yunzhi.,...&Yuan, Zhiming.(2019).Characterization of a Novel Tanay Virus Isolated From Anopheles sinensis Mosquitoes in Yunnan, China.FRONTIERS IN MICROBIOLOGY,10,11. |
| MLA | Zhao, Lu,et al."Characterization of a Novel Tanay Virus Isolated From Anopheles sinensis Mosquitoes in Yunnan, China".FRONTIERS IN MICROBIOLOGY 10(2019):11. |
入库方式： OAI收割
来源：武汉植物园
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