Catechins-Modified Selenium-Doped Hydroxyapatite Nanomaterials for Improved Osteosarcoma Therapy Through Generation of Reactive Oxygen Species
文献类型：期刊论文
| 作者 | Khan, Suliman2,3,4; Ullah, Muhammad Wajid5; Siddique, Rabeea2,5; Liu, Yang1,2; Ullah, Ismat6; Xue, Mengzhou1,2; Yang, Guang5; Hou, Hongwei3,4 |
| 刊名 | FRONTIERS IN ONCOLOGY
 |
| 出版日期 | 2019-06-13 |
| 卷号 | 9页码:13 |
| 关键词 | osteosarcoma catechins selenium hydroxyapatite ROS cancer therapy |
| ISSN号 | 2234-943X |
| DOI | 10.3389/fonc.2019.00499 |
| 英文摘要 | Osteosarcoma is the most common bone cancer with limited therapeutic options. It can be treated by selenium-doped hydroxyapatite owing to its known antitumor potential. However, a high concentration of Se is toxic toward normal and stem cells whereas its low concentration cannot effectively remove cancer cells. Therefore, the current study was aimed to improve the anticancer activity of Se-HAp nanoparticles through catechins (CC) modification owing to their high cancer therapeutic value. The sequentially developed catechins modified Se-HAp nanocomposites (CC/Se-HAp) were characterized for various physico-chemical properties and antitumor activity. Structural analysis showed the synthesis of small rod-like single phase HAp nanoparticles (60 +/- 15 nm), which effectively interacted with Se and catechins and formed agglomerated structures. TEM analysis showed the internalization and degradation of CC/Se-HAp nanomaterials within MNNG/HOS cells through a non-specific endocytosis process. Cell toxicity analysis showed that catechins modification improved the antitumor activity of Se-HAp nanocomposites by inducing apoptosis of human osteosarcoma MNNG/HOS cell lines, through generation of reactive oxygen species (ROS) which in turn activated the caspase-3 pathway, without significantly affecting the growth of human normal bone marrow stem cells (hBMSCs). qPCR and western blot analyses revealed that casp3, p53, and bax genes were significantly upregulated while cox-2 and PTK-2 were slightly downregulated as compared to control in CC/Se-HAp-treated MNNG/HOS cell lines. The current study of combining natural biomaterial (i.e., catechins) with Se and HAp, can prove to be an effective therapeutic approach for bone cancer therapy. |
| 资助项目 | National Science Foundation of China[31870917] ; National Science Foundation of China[81471174] ; National Science Foundation of China[81520108011] ; National Science Foundation of China[81870942] ; National Key Research and Development Program of China[2018YFC1312200] ; Innovation Scientists and Technicians Troop Constructions Projects of Henan Province of China ; China Postdoctoral Science Foundation[2016M602291] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000471845900001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8148]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Xue, Mengzhou; Yang, Guang; Hou, Hongwei |
| 作者单位 | 1.Henan Med Key Lab Translat Cerebrovasc Dis, Zhengzhou, Henan, Peoples R China 2.Zhengzhou Univ, Affiliated Hosp 2, Dept Cerebrovasc Dis, Zhengzhou, Henan, Peoples R China 3.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Huazhong Univ Sci & Technol, Dept Biomed Engn, Wuhan, Hubei, Peoples R China 6.Huazhong Univ Sci & Technol, State Key Lab Mat Proc & Die Mold Technol, Sch Mat Sci & Engn, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Khan, Suliman,Ullah, Muhammad Wajid,Siddique, Rabeea,et al. Catechins-Modified Selenium-Doped Hydroxyapatite Nanomaterials for Improved Osteosarcoma Therapy Through Generation of Reactive Oxygen Species[J]. FRONTIERS IN ONCOLOGY,2019,9:13. |
| APA | Khan, Suliman.,Ullah, Muhammad Wajid.,Siddique, Rabeea.,Liu, Yang.,Ullah, Ismat.,...&Hou, Hongwei.(2019).Catechins-Modified Selenium-Doped Hydroxyapatite Nanomaterials for Improved Osteosarcoma Therapy Through Generation of Reactive Oxygen Species.FRONTIERS IN ONCOLOGY,9,13. |
| MLA | Khan, Suliman,et al."Catechins-Modified Selenium-Doped Hydroxyapatite Nanomaterials for Improved Osteosarcoma Therapy Through Generation of Reactive Oxygen Species".FRONTIERS IN ONCOLOGY 9(2019):13. |
入库方式： OAI收割
来源：武汉植物园
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