中国科学院机构知识库网格系统: Expression and Regulation of PpEIN3b during Fruit Ripening and Senescence via Integrating SA, Glucose, and ACC Signaling in Pear (Pyrus pyrifolia Nakai. Whangkeumbae)

Expression and Regulation of PpEIN3b during Fruit Ripening and Senescence via Integrating SA, Glucose, and ACC Signaling in Pear (Pyrus pyrifolia Nakai. Whangkeumbae)

文献类型：期刊论文


作者	Shi, Haiyan 1; Zhang, Yuxing 1; Chen, Liang 2
刊名	GENES
出版日期	2019-06-01
卷号	10 期号:6 页码:11
关键词	pear (Pyrus pyrifolia Nakai Whangkeumbae) ethylene insensitive3 salicylic acid gene expression fruit ripening and senescence
ISSN号	2073-4425
DOI	10.3390/genes10060476
英文摘要	The economic value of fruit is reduced by having a short shelf life. Whangkeumbae is a type of sand pear (Pyrus pyrifolia) considered a climacteric fruit. The pear is famous for its smooth surface and good flavor. However, its shelf life is very short because of senescence and disease after harvest and a burst of ethylene (ET) production prompting the onset of fruit ripening. In plants, ETHYLENE INSENSITIVE3 (EIN3) and EIN3like (EIL), located in the nucleus, are important components of the ET signaling pathway and act as transcription factors. EIN3s and EILs belong to a small family involved in regulating the expression of ethylene response factor gene (ERF), whose encoding protein is the final component in the ET signaling pathway. The mutation of these components will cause defects in the ethylene pathway. In this study, one gene encoding an EIN3 was cloned and identified from Whangkeumbae and designated PpEIN3b. The deduced PpEIN3b contained a conserved EIN3 domain, a bipartite nuclear localization signal profile (NLS_BP), and an N-6 adenine-specific DNA methylase signature (N6_MTASE). PpEIN3b belongs to the EIN3 super-family by phylogenetic analysis. Quantitative RT-PCR (qRT-PCR) analysis revealed that PpEIN3b was preferentially expressed in fruit. Additionally, its expression was developmentally regulated during fruit ripening and senescence. Furthermore, PpEIN3b transcripts were obviously repressed by salicylic acid (SA) and glucose treatment in pear fruit and in diseased fruit, while it was significantly induced by 1-aminocyclopropane-1-carboxylic acid (ACC) treatment. Taken together, our results reveal the expression and regulation profiles of PpEIN3b and suggest that PpEIN3b might integrate SA, glucose, and ACC signaling to regulate fruit ripening and senescence in pear, which would provide a candidate gene for this regulation to obtain fruit with a long shelf life and improved economic value.
资助项目	National Natural Sciences Foundation of China[31301761] ; Education Department of Hebei Province Foundation[BJ2014032] ; Top-Notch Talent of Hebei Province
WOS研究方向	Genetics & Heredity
语种	英语
WOS记录号	WOS:000473797000071
出版者	MDPI
源URL	[http://202.127.146.157/handle/2RYDP1HH/8208]
专题	中国科学院武汉植物园
通讯作者	Shi, Haiyan; Chen, Liang
作者单位	1.Agr Univ Hebei, Coll Hort, Pear Engn & Technol Res Ctr Hebei, Baoding 071001, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Shi, Haiyan,Zhang, Yuxing,Chen, Liang. Expression and Regulation of PpEIN3b during Fruit Ripening and Senescence via Integrating SA, Glucose, and ACC Signaling in Pear (Pyrus pyrifolia Nakai. Whangkeumbae)[J]. GENES,2019,10(6):11.
APA	Shi, Haiyan,Zhang, Yuxing,&Chen, Liang.(2019).Expression and Regulation of PpEIN3b during Fruit Ripening and Senescence via Integrating SA, Glucose, and ACC Signaling in Pear (Pyrus pyrifolia Nakai. Whangkeumbae).GENES,10(6),11.
MLA	Shi, Haiyan,et al."Expression and Regulation of PpEIN3b during Fruit Ripening and Senescence via Integrating SA, Glucose, and ACC Signaling in Pear (Pyrus pyrifolia Nakai. Whangkeumbae)".GENES 10.6(2019):11.

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来源：武汉植物园

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Expression and Regulation of PpEIN3b during Fruit Ripening and Senescence via Integrating SA, Glucose, and ACC Signaling in Pear (Pyrus pyrifolia Nakai. Whangkeumbae)

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