Enrichment and analysis of quaternary alkaloids from Zanthoxylum simulans using weak cation exchange solid-phase extraction coupled with LC-MS
文献类型：期刊论文
| 作者 | Fan, Minxia2,3; Tian, Yongqiang2,4; Chen, Guilin1,2; Sarker, Satyajit D.5; Nahar, Lutfun5; Wu, Jianlin6; Li, Na6; Guo, Mingquan1,2 |
| 刊名 | PHYTOCHEMICAL ANALYSIS
 |
| 出版日期 | 2019-07-17 |
| 页码 | 8 |
| 关键词 | quaternary alkaloids solid-phase extraction weak cation exchange Zanthoxylum simulans |
| ISSN号 | 0958-0344 |
| DOI | 10.1002/pca.2860 |
| 英文摘要 | Introduction Quaternary alkaloids (QAs) are the major alkaloids in several traditional Chinese medicines, especially in Zanthoxylum simulans. However, few studies on enrichment of QAs from Z. simulans were conducted due to their high polarity and low content. Objective To develop a weak cation exchange (WCX) solid-phase extraction (SPE) coupled with liquid chromatography mass spectrometry (LC-MS) method to enrich and identify QAs from Z. simulans. Meanwhile, the qualitative and quantitative analyses of QAs were carried out based on the optimum conditions of the method. Methods Fresh stem bark of Z. simulans was extracted with 70% aqueous methanol and enriched by WCX-SPE. A high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) with an electrospray ionisation (ESI) source was used for the qualitative and quantitative analyses of QAs. Results Significant improvements were observed in resolution and abundance of the peaks with WCX-SPE. The linearity, limit of detection (LOD) and limit of quantification (LOQ) were determined for this analytical method. The linear relationship (A = 338.85C - 187.72, R-2 = 0.99) was explored in the range of 0.5 to 312.5 mu g/mL for chelerythrine. The LOD and LOQ for chelerythrine standard solutions were 0.0539 mu g/mL and 0.1798 mu g/mL, respectively. In addition, 22 peaks were detected successfully with WCX-SPE and nine of them are undetectable without the processing of WCX-SPE. Conclusion A highly selective and efficient method for simultaneous enrichment and identification of QAs from crude extract of Z. simulans was developed for the first time by combining WCX-SPE with LC-MS. |
| 资助项目 | Natural Science Foundation of Hubei Province[2017CFB186] ; Macao Science and Technology Development Fund[0090/2018/A3] ; Major Project for Special Technology Innovation of Hubei Province[2017AHB054] ; National Natural Science Foundation of China[81673580] ; National Natural Science Foundation of China[81803676] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000476206000001 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8406]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Mingquan |
| 作者单位 | 1.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Wuhan Hosp Tradit Chinese Med, Wuhan, Hubei, Peoples R China 5.Liverpool John Moores Univ, Ctr Nat Prod Discovery, Sch Pharm & Biomol Sci, Liverpool, Merseyside, England 6.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa, Macao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Minxia,Tian, Yongqiang,Chen, Guilin,et al. Enrichment and analysis of quaternary alkaloids from Zanthoxylum simulans using weak cation exchange solid-phase extraction coupled with LC-MS[J]. PHYTOCHEMICAL ANALYSIS,2019:8. |
| APA | Fan, Minxia.,Tian, Yongqiang.,Chen, Guilin.,Sarker, Satyajit D..,Nahar, Lutfun.,...&Guo, Mingquan.(2019).Enrichment and analysis of quaternary alkaloids from Zanthoxylum simulans using weak cation exchange solid-phase extraction coupled with LC-MS.PHYTOCHEMICAL ANALYSIS,8. |
| MLA | Fan, Minxia,et al."Enrichment and analysis of quaternary alkaloids from Zanthoxylum simulans using weak cation exchange solid-phase extraction coupled with LC-MS".PHYTOCHEMICAL ANALYSIS (2019):8. |
入库方式： OAI收割
来源：武汉植物园
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