Toll-Like Receptor 7 Activation Enhances CD8+T Cell Effector Functions by Promoting Cellular Glycolysis
文献类型：期刊论文
| 作者 | Li, Qian5; Yang, Yan2; Liu, Jia3,5; Huang, Xuan4,6; Zhang, Xiaoyong4,6; Kirschning, Carsten7; Xu, Haifeng C.8; Lang, Philipp A.8; Dittmar, Ulf5; Zhang, Ejuan1,5 |
| 刊名 | FRONTIERS IN IMMUNOLOGY
 |
| 出版日期 | 2019-09-12 |
| 卷号 | 10页码:14 |
| 关键词 | toll-like receptor 7 CD8+T cells PI3K-Akt-mTOR glycolysis IRF4 |
| ISSN号 | 1664-3224 |
| DOI | 10.3389/fimmu.2019.02191 |
| 英文摘要 | The activation of TLR7 signaling in T cells accelerates antigen-specific responses. Such responses play an essential role in eliminating viral infections and can be anti-tumorigenic. However, the underlying mechanisms of how TLR7 can promote the optimal function of CD8+ T cells remain unclear. To investigate how TLR signaling directly contributes to CD8+ T cell functions, we examine the activation of cellular TLR7-related pathways and functional and metabolic alterations in TLR7-stimulated T cells during T cell receptor (TCR) signaling. In the present study, we investigated the activation of CD8+ T cells in response to direct stimulation by TLR7 ligands. TLR7 stimulation could promote the effector functions of purified CD8+ T cells in vitro. The TLR7-induced activation of CD8+ T cells occurs if CD8+ T cells were primed by alpha CD3 activation and increasingly expressed TLR7. MyD88 and AKT-mTOR signaling plays a critical role in TLR7-induced T cell activation. In addition to the upregulation of immune-related genes, metabolic alterations in CD8+ T cells, including the upregulation of glucose uptake and glycolysis, occurred by TLR7 stimulation. Glycolysis was found to be regulated by the AKT-mTOR pathway and a downstream transcription factor IRF4. Blocking glycolysis by either direct glucose deprivation or modulating the mTOR pathway and IRF4 expression was found to impair T cell activation and functions. Taken together, the activation of TLR7 signaling promotes the effector functions of CD8+ T cells by enhancing cellular glycolysis. |
| 资助项目 | Deutsche Forschungsgemeinschaft[RTG1949/1] ; Deutsche Forschungsgemeinschaft[Transregio TRR60] ; National Natural Science Foundation of China[81701550] ; National Natural Science Foundation of China[81771688] ; German Research Council[SFB974] ; German Research Council[LA2558/5-1] ; German Research Council[RTG1949] ; Jurgen Manchot Graduate School (MOI III) |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000485282500001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8408]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Ejuan; Lui, Mengji |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Mucosa Immun Res Grp, Wuhan, Hubei, Peoples R China 2.Jiangnan Univ, Affiliated Hosp, Peoples Hosp Wuxi 5, Ctr Clin Lab, Wuxi, Jiangsu, Peoples R China 3.Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Infect Dis, Wuhan, Hubei, Peoples R China 4.Southern Med Univ, Nanfang Hosp, Hepatol Unit, Guangzhou, Guangdong, Peoples R China 5.Univ Duisburg Essen, Univ Hosp Essen, Inst Virol, Essen, Germany 6.Southern Med Univ, Nanfang Hosp, Dept Infect Dis, Guangzhou, Guangdong, Peoples R China 7.Univ Duisburg Essen, Univ Hosp Essen, Inst Med Microbiol, Essen, Germany 8.Heinrich Heine Univ, Med Fac, Dept Mol Med 2, Dusseldorf, Germany |
| 推荐引用方式 GB/T 7714 | Li, Qian,Yang, Yan,Liu, Jia,et al. Toll-Like Receptor 7 Activation Enhances CD8+T Cell Effector Functions by Promoting Cellular Glycolysis[J]. FRONTIERS IN IMMUNOLOGY,2019,10:14. |
| APA | Li, Qian.,Yang, Yan.,Liu, Jia.,Huang, Xuan.,Zhang, Xiaoyong.,...&Lui, Mengji.(2019).Toll-Like Receptor 7 Activation Enhances CD8+T Cell Effector Functions by Promoting Cellular Glycolysis.FRONTIERS IN IMMUNOLOGY,10,14. |
| MLA | Li, Qian,et al."Toll-Like Receptor 7 Activation Enhances CD8+T Cell Effector Functions by Promoting Cellular Glycolysis".FRONTIERS IN IMMUNOLOGY 10(2019):14. |
入库方式： OAI收割
来源：武汉植物园
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