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Evolution of l-DOPA 4,5-dioxygenase activity allows for recurrent specialisation to betalain pigmentation in Caryophyllales

文献类型：期刊论文

作者Sheehan, Hester2; Feng, Tao2,3; Walker-Hale, Nathanael2; Lopez-Nieves, Samuel2; Pucker, Boas2,4,5; Guo, Rui2,3,6; Yim, Won C.1; Badgami, Roshani2; Timoneda, Alfonso2; Zhao, Lijun7
刊名NEW PHYTOLOGIST
出版日期2019-09-29
页码16
关键词anthocyanins betalains Caryophyllales convergent evolution gene duplication l-DOPA 4 5-dioxygenase (DODA) metabolic operon plant pigments specialised metabolism
ISSN号0028-646X
DOI10.1111/nph.16089
英文摘要The evolution of l-DOPA 4,5-dioxygenase activity, encoded by the gene DODA, was a key step in the origin of betalain biosynthesis in Caryophyllales. We previously proposed that l-DOPA 4,5-dioxygenase activity evolved via a single Caryophyllales-specific neofunctionalisation event within the DODA gene lineage. However, this neofunctionalisation event has not been confirmed and the DODA gene lineage exhibits numerous gene duplication events, whose evolutionary significance is unclear. To address this, we functionally characterised 23 distinct DODA proteins for l-DOPA 4,5-dioxygenase activity, from four betalain-pigmented and five anthocyanin-pigmented species, representing key evolutionary transitions across Caryophyllales. By mapping these functional data to an updated DODA phylogeny, we then explored the evolution of l-DOPA 4,5-dioxygenase activity. We find that low l-DOPA 4,5-dioxygenase activity is distributed across the DODA gene lineage. In this context, repeated gene duplication events within the DODA gene lineage give rise to polyphyletic occurrences of elevated l-DOPA 4,5-dioxygenase activity, accompanied by convergent shifts in key functional residues and distinct genomic patterns of micro-synteny. In the context of an updated organismal phylogeny and newly inferred pigment reconstructions, we argue that repeated convergent acquisition of elevated l-DOPA 4,5-dioxygenase activity is consistent with recurrent specialisation to betalain synthesis in Caryophyllales.
资助项目BBSRC High Value Chemicals from Plants Network ; SNF[P2BEP3_165359] ; SNF[P300PA_174333] ; Woolf Fisher Cambridge Scholarship ; NSF DEB[1352907] ; NSF DEB[1354048] ; DOE[GSP DE-SC0008834] ; CSC[(2018) 3101] ; [U1802232] ; [XDA20050203]
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000487927800001
出版者WILEY
源URL[http://202.127.146.157/handle/2RYDP1HH/8418]  
专题中国科学院武汉植物园
通讯作者Brockington, Samuel F.
作者单位1.Univ Nevada, Dept Biochem & Mol Biol, Reno, NV 89577 USA
2.Univ Cambridge, Dept Plant Sci, Tennis Court Rd, Cambridge CB2 3EA, England
3.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan 430074, Hubei, Peoples R China
4.Bielefeld Univ, CeBiTec, Univ Str, D-33615 Bielefeld, Germany
5.Bielefeld Univ, Fac Biol, Univ Str, D-33615 Bielefeld, Germany
6.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
7.Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA
8.Oberlin Coll, Dept Biol, Sci Ctr K111, Oberlin, OH 44074 USA
9.Univ Arizona, Sch Plant Sci, Arizona Genom Inst, Tucson, AZ 85721 USA
10.Swiss Fed Inst Technol, Inst Agr Sci, Mol Plant Breeding, Univ Str 2, CH-8092 Zurich, Switzerland
推荐引用方式
GB/T 7714		 Sheehan, Hester,Feng, Tao,Walker-Hale, Nathanael,et al. Evolution of l-DOPA 4,5-dioxygenase activity allows for recurrent specialisation to betalain pigmentation in Caryophyllales[J]. NEW PHYTOLOGIST,2019:16.
APA Sheehan, Hester.,Feng, Tao.,Walker-Hale, Nathanael.,Lopez-Nieves, Samuel.,Pucker, Boas.,...&Brockington, Samuel F..(2019).Evolution of l-DOPA 4,5-dioxygenase activity allows for recurrent specialisation to betalain pigmentation in Caryophyllales.NEW PHYTOLOGIST,16.
MLA Sheehan, Hester,et al."Evolution of l-DOPA 4,5-dioxygenase activity allows for recurrent specialisation to betalain pigmentation in Caryophyllales".NEW PHYTOLOGIST (2019):16.

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