Quantum Dot Nanobeacons for Single RNA Labeling and Imaging
文献类型：期刊论文
| 作者 | Ma, Yingxin2,3; Mao, Guobin1; Huang, Weiren3; Wu, Guoqiang3; Yin, Wen2; Ji, Xinghu1; Deng, Zishi3; Cai, Zhiming3; Zhang, Xian-En4; He, Zhike1 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2019-08-28 |
| 卷号 | 141期号:34页码:13454-13458 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.9b04659 |
| 英文摘要 | Detection and imaging RNAs in live cells is in high demand. Methodology for such a purpose is still a challenge, particularly for single RNA detection and imaging in live cells. In this study, a type of quantum dot (QD) nanobeacon with controllable valencies was constructed by precisely conjugating the black hole quencher (BHQ1) and phosphorothioate comodified DNA onto CdTe:Zn2+ QDs via a one-pot hydrothermal method. The nanobeacon with only one conjugated DNA was used to label and detect low-abundance nucleic acids in live cells, and single HIV-1 RNAs were detected and imaged in live HIV-1 integrated cells. Additionally, QD nanobeacon-labeled HIV-1 genomic RNAs were encapsulated in progeny viral particles, which can be used to track the uncoating process of single viruses. The current study provides a platform for nucleic acid labeling and imaging with high sensitivity, being especially meaningful for tracking of individual RNAs in live cells. |
| 资助项目 | Strategic Priority Research Program of Chinese Academy of Sciences[XDB29050201] ; National Natural Science Foundation of China[21675119] ; National Natural Science Foundation of China[21705110] ; National Natural Science Foundation of China[81772737] ; National Major Science and Technology Projects[2018ZX10301405] ; Natural Science Foundation of Guangdong[2018B030306046] ; China Postdoctoral Science Foundation[BX201700161] ; China Postdoctoral Science Foundation[2017M622860] ; Shenzhen Municipal Government of China[JCYJ20170413161749433] ; Shenzhen Municipal Government of China[JSGG20160301161836370] ; Sanming Project of Shenzhen Health and Family Planning Commission[SZSM201412018] ; Sanming Project of Shenzhen Health and Family Planning Commission[SZSM201512037] ; High Level University's Medical Discipline Construction[2016031638] ; Shenzhen Health and Family Planning Commission[201606019] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000484082700024 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8493]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | He, Zhike; Cui, Zongqiang |
| 作者单位 | 1.Wuhan Univ, Coll Chem & Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China 3.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Key Lab Med Reprogramming Technol,Sch Med, Shenzhen, Peoples R China 4.Chinese Acad Sci, Inst Biophys, CAS Ctr Biol Macromol, Natl Key Lab Biomacromol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Yingxin,Mao, Guobin,Huang, Weiren,et al. Quantum Dot Nanobeacons for Single RNA Labeling and Imaging[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2019,141(34):13454-13458. |
| APA | Ma, Yingxin.,Mao, Guobin.,Huang, Weiren.,Wu, Guoqiang.,Yin, Wen.,...&Cui, Zongqiang.(2019).Quantum Dot Nanobeacons for Single RNA Labeling and Imaging.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,141(34),13454-13458. |
| MLA | Ma, Yingxin,et al."Quantum Dot Nanobeacons for Single RNA Labeling and Imaging".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 141.34(2019):13454-13458. |
入库方式： OAI收割
来源：武汉植物园
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