Recurrent somatic embryogenesis and development of somatic embryos in Akebia trifoliata (Thunb.) Koidz (Lardizabalaceae)
文献类型：期刊论文
| 作者 | Zou, Shuaiyu1,2,3; Yao, Xiaohong4; Zhong, Caihong4; Li, Dawei4; Wang, Zupeng4; Huang, Hongwen2,3 |
| 刊名 | PLANT CELL TISSUE AND ORGAN CULTURE
 |
| 出版日期 | 2019-10-11 |
| 页码 | 12 |
| 关键词 | Akebia trifoliata Somatic embryogenesis Somatic embryos Development Hormone-free medium |
| ISSN号 | 0167-6857 |
| DOI | 10.1007/s11240-019-01686-4 |
| 英文摘要 | A simple and effective protocol was established for recurrent somatic embryogenesis and plant regeneration in Akebia trifoliata (Thunb.) Koidz. Somatic embryos directly formed from the root zone of the immature zygotic embryos cultured on MS medium devoid of plant growth regulator (PGR). The induction frequency of immature zygotic embryos was 52.5%, and the mean number of somatic embryos reached up to 19.5. Secondary somatic embryos arose on the primary embryos at a high frequency (95.8%), and this process maintained in a recurrent way on PGR-free MS medium. The highest mean number of somatic embryos also appeared in the primary embryos which can reach up to 44.7. While both of the embryogenic potential and mean number of embryos per explant displayed a gradual diminution with subculturing. The addition of 0.5 mg l(-1) 6-benzyladenine in the 1/2 MS regeneration medium can significantly improve the frequency of somatic embryos convert to plantlets. The microscopic analysis revealed that the development process of somatic embryos via the globular, heart, torpedo and cotyledonal stages in A. trifoliata, the histological analysis showed that somatic embryos directly initiated from root epidermal cells and there are no vascular connections between the somatic embryos and maternal tissue. Regenerated plantlets acclimated successfully to greenhouse conditions. Approximately 65% plantlets survived and displayed no morphological characteristics differences with seed-derived plants. The simple and effective protocols established in this study will promote large-scale clonal propagation and genetic improvement of A. trifoliata. Key message Somatic embryogenesis has never been reported in Akebia trifoliata (Thunb.) Koidz. The study showed the origin and development process of somatic embryos in Akebia trifoliata (Thunb.) Koidz. |
| 资助项目 | Plant Germplasm Innovation Program, Science and Technology Service Network Initiative, Chinese Academy of Sciences[ZSZC-007] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000490038400002 |
| 出版者 | SPRINGER |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8538]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Huang, Hongwen |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China 3.Guangdong Prov Key Lab Appl Bot, Guangzhou 510650, Guangdong, Peoples R China 4.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Shuaiyu,Yao, Xiaohong,Zhong, Caihong,et al. Recurrent somatic embryogenesis and development of somatic embryos in Akebia trifoliata (Thunb.) Koidz (Lardizabalaceae)[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2019:12. |
| APA | Zou, Shuaiyu,Yao, Xiaohong,Zhong, Caihong,Li, Dawei,Wang, Zupeng,&Huang, Hongwen.(2019).Recurrent somatic embryogenesis and development of somatic embryos in Akebia trifoliata (Thunb.) Koidz (Lardizabalaceae).PLANT CELL TISSUE AND ORGAN CULTURE,12. |
| MLA | Zou, Shuaiyu,et al."Recurrent somatic embryogenesis and development of somatic embryos in Akebia trifoliata (Thunb.) Koidz (Lardizabalaceae)".PLANT CELL TISSUE AND ORGAN CULTURE (2019):12. |
入库方式： OAI收割
来源：武汉植物园
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