Interaction between Silver Nanoparticles and Two Dehydrogenases: Role of Thiol Groups
文献类型：期刊论文
| 作者 | Jiang, Hong Sheng5,6; Zhang, Yizhi1,6; Lu, Zhen Wei2,5; Lebrun, Regine3; Gontero, Brigitte6; Li, Wei4,5 |
| 刊名 | SMALL
 |
| 出版日期 | 2019-07-01 |
| 卷号 | 15期号:27页码:12 |
| 关键词 | enzyme activity GAPDH MDH silver nanoparticles thiol groups |
| ISSN号 | 1613-6810 |
| DOI | 10.1002/smll.201900860 |
| 英文摘要 | Widely used silver nanoparticles (AgNPs) are readily accessible to biological fluids and then surrounded by proteins. However, interactions between AgNPs and proteins are poorly understood. Two dehydrogenases, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and malate dehydrogenase (MDH), are chosen to investigate these interactions. Ag bound to thiol groups of these enzymes significantly decreases the number of free thiols available. Dose-dependent inhibition of enzyme activities is observed in both AgNPs and Ag+ treatments. Based on the concentration required to inhibit 50% activity, GAPDH and MDH are 24-30 fold more sensitive to Ag+ than to AgNPs suggesting that the measured 4.2% Ag+ containing AgNPs can be responsible for the enzymes inhibition. GAPDH, with a thiol group in its active site, is more sensitive to Ag than MDH, displaying many thiol groups but none in its active site, suggesting that thiol groups at the active site strongly determines the sensitivity of enzymes toward AgNPs. In contrast, the dramatic changes of circular dichroism spectra show that the global secondary structure of MDH under AgNPs treatment is more altered than that of GAPDH. In summary, this study shows that the thiol groups and their location on these dehydrogenases are crucial for the AgNPs effects. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDB31010000] ; Aix Marseille Univ ; China Scholarship Council (CSC) ; Chinese Academy of Sciences[2016VBA006] ; CNRS |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000477930000010 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8682]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Gontero, Brigitte; Li, Wei |
| 作者单位 | 1.Yunnan Univ, Ctr Life Sci, Sch Life Sci, Kunming 6500091, Yunnan, Peoples R China 2.Hainan Univ, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou 570228, Hainan, Peoples R China 3.CNRS, Plate Forme Proteom, Marseille Proteom MaP, IMM,FR 3479, 31 Chemin J Aiguier, F-13009 Marseille, France 4.Chinese Acad Sci, Wuhan Bot Garden, Hubei Key Lab Wetland Evolut & Ecol Restorat, Wuhan 430074, Hubei, Peoples R China 5.Chinese Acad Sci, Core Bot Gardens, Wuhan Bot Garden & Ctr Plant Ecol, Key Lab Aquat Bot & Watershed Ecol, Wuhan 430074, Hubei, Peoples R China 6.Aix Marseille Univ, CNRS, BIP UMR 7281, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France |
| 推荐引用方式 GB/T 7714 | Jiang, Hong Sheng,Zhang, Yizhi,Lu, Zhen Wei,et al. Interaction between Silver Nanoparticles and Two Dehydrogenases: Role of Thiol Groups[J]. SMALL,2019,15(27):12. |
| APA | Jiang, Hong Sheng,Zhang, Yizhi,Lu, Zhen Wei,Lebrun, Regine,Gontero, Brigitte,&Li, Wei.(2019).Interaction between Silver Nanoparticles and Two Dehydrogenases: Role of Thiol Groups.SMALL,15(27),12. |
| MLA | Jiang, Hong Sheng,et al."Interaction between Silver Nanoparticles and Two Dehydrogenases: Role of Thiol Groups".SMALL 15.27(2019):12. |
入库方式： OAI收割
来源：武汉植物园
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