Testing and using complete plastomes and ribosomal DNA sequences as the next generation DNA barcodes in Panax (Araliaceae)
文献类型：期刊论文
| 作者 | Ji, Yunheng2,3; Liu, Changkun2; Yang, Zhenyan2; Yang, Lifang2,4; He, Zhengshan5; Wang, Hengchang1; Yang, Junbo5; Yi, Tingshuang5 |
| 刊名 | MOLECULAR ECOLOGY RESOURCES
 |
| 出版日期 | 2019-07-25 |
| 页码 | 13 |
| 关键词 | next-generation DNA barcodes Panax plastome ribosomal DNA species discrimination standard DNA barcodes |
| ISSN号 | 1755-098X |
| DOI | 10.1111/1755-0998.13050 |
| 英文摘要 | Complete plastid genome (plastome) sequences and nuclear ribosomal DNA (nrDNA) regions have been proposed as candidates for the next generation of DNA barcodes for plant species discrimination. However, the efficacy of this approach still lacks comprehensive evaluation. We carried out a case study in the economically important but phylogenetically and taxonomically difficult genus Panax (Araliaceae). We generated a large data set of plastomes and nrDNA sequences from multiple accessions per species. Our data improved the phylogenetic resolution and levels of species discrimination in Panax, compared to any previous studies using standard DNA barcodes. This provides new insights into the speciation, lineage diversification and biogeography of the genus. However, both plastome and nrDNA failed to completely resolve the phylogenetic relationships in the Panax bipinnatifidus species complex, and only half of the species within it were recovered as monophyletic units. The results suggest that complete plastome and ribosomal DNA sequences can substantially increase species discriminatory power in plants, but they are not powerful enough to fully resolve phylogenetic relationships and discriminate all species, particularly in evolutionarily young and complex plant groups. To gain further resolving power for closely related species, the addition of substantial numbers of nuclear markers is likely to be required. |
| 资助项目 | Major Program of National Natural Science Foundation of China[31590820] ; Major Program of National Natural Science Foundation of China[31590823] ; National Natural Science Foundation of China[31872673] ; Chinese Academy of Sciences the Large-scale Scientific Facilities[2017-LSF-GBOWS-02] ; NSFC-Joint Foundation of Yunnan Province[U1802287] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000479384000001 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8718]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Ji, Yunheng; Yang, Junbo; Yi, Tingshuang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming Inst Bot, Kunming, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Integrat Conservat Plant Species E, Kunming, Yunnan, Peoples R China 4.Yunnan Univ, Sch Life Sci, Kunming, Yunnan, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Yunheng,Liu, Changkun,Yang, Zhenyan,et al. Testing and using complete plastomes and ribosomal DNA sequences as the next generation DNA barcodes in Panax (Araliaceae)[J]. MOLECULAR ECOLOGY RESOURCES,2019:13. |
| APA | Ji, Yunheng.,Liu, Changkun.,Yang, Zhenyan.,Yang, Lifang.,He, Zhengshan.,...&Yi, Tingshuang.(2019).Testing and using complete plastomes and ribosomal DNA sequences as the next generation DNA barcodes in Panax (Araliaceae).MOLECULAR ECOLOGY RESOURCES,13. |
| MLA | Ji, Yunheng,et al."Testing and using complete plastomes and ribosomal DNA sequences as the next generation DNA barcodes in Panax (Araliaceae)".MOLECULAR ECOLOGY RESOURCES (2019):13. |
入库方式： OAI收割
来源：武汉植物园
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