The level of genetic diversity and differentiation of tropical lotus, Nelumbo nucifera Gaertn. (Nelumbonaceae) from Australia, India, and Thailand
文献类型：期刊论文
| 作者 | Mekbib,Yeshitila1,2,3,4; Huang,Shi-Xu5; Ngarega,Boniface K.1,2,3; Li,Zhi-Zhong1,2,3; Shi,Tao1,2; Ou,Ke-Fang6; Liang,Yu-Ting6; Chen,Jin-Ming1,2; Yang,Xing-Yu6 |
| 刊名 | Botanical Studies
 |
| 出版日期 | 2020-05-16 |
| 卷号 | 61期号:1 |
| 关键词 | Conservation Genetic diversity Gene flow Nelumbo nucifera SSR markers Tropical lotus |
| DOI | 10.1186/s40529-020-00293-3 |
| 英文摘要 | AbstractBackgroundNelumbo nucifera Gaertn., a perennial aquatic macrophyte species, has been cultivated in several Asian countries for its economic importance, and medicinal uses. Two distinct ecotypes of the species are recognized based on the geographical location where the genotypes are adapted, i.e., tropical lotus and temperate lotus. The genetic diversity levels and differentiation of the tropical lotus from poorly studied geographic regions still remain unclear. Here, the population genetic diversity and structure of 15 tropical lotus populations sampled from the previous understudied natural distribution ranges, including India, Thailand, and Australia, were assessed using nine polymorphic SSR markers.ResultsThe SSR markers used to genotype the 216 individuals yielded 65 alleles. The highest and lowest genetic diversity estimates were found in Thailand and Indian populations, respectively. STRUCTURE analysis revealed three distinct genetic clusters, with relatively low admixtures, supported by PCoA cluster analysis. Low levels of gene flow (mean N?m?=?0.346) among the three genetic clusters signified the Mantel test for isolation by distance, revealing the existence of a positive correlation between the genetic and geographic distances (r?=?0.448, P?=?0.004). Besides, AMOVA analysis revealed a higher variation among populations (59.98%) of the three groups. Overall, the populations used in this study exposed a high level of genetic differentiation (FST?=?0.596).ConclusionsThe nine polymorphic microsatellite markers used in our study sufficiently differentiated the fifteen tropical?N. nucifera?populations based on geography. These populations presented different genetic variability, thereby confirming that populations found in each country are unique. The low genetic diversity (HE?=?0.245) could be explained by limited gene flow and clonal propagation. Conserving the available diversity using various conservation approaches is essential to enable the continued utilization of this economically important crop species. We, therefore, propose that complementary conservation approaches ought to be introduced to conserve tropical lotus, depending on the genetic variations and threat levels in populations. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/S40529-020-00293-3 |
| 出版者 | Springer Singapore |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14356]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chen,Jin-Ming; Yang,Xing-Yu |
| 作者单位 | 1.Chinese Academy of Sciences; CAS Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden 2.Chinese Academy of Sciences; Center of Conservation Biology, Core Botanical Gardens 3.University of Chinese Academy of Sciences 4.Ethiopian Biodiversity Institute 5.South-Central University for Nationalities; College of Life Science 6.Wuhan Institute of Landscape Architecture |
| 推荐引用方式 GB/T 7714 | Mekbib,Yeshitila,Huang,Shi-Xu,Ngarega,Boniface K.,et al. The level of genetic diversity and differentiation of tropical lotus, Nelumbo nucifera Gaertn. (Nelumbonaceae) from Australia, India, and Thailand[J]. Botanical Studies,2020,61(1). |
| APA | Mekbib,Yeshitila.,Huang,Shi-Xu.,Ngarega,Boniface K..,Li,Zhi-Zhong.,Shi,Tao.,...&Yang,Xing-Yu.(2020).The level of genetic diversity and differentiation of tropical lotus, Nelumbo nucifera Gaertn. (Nelumbonaceae) from Australia, India, and Thailand.Botanical Studies,61(1). |
| MLA | Mekbib,Yeshitila,et al."The level of genetic diversity and differentiation of tropical lotus, Nelumbo nucifera Gaertn. (Nelumbonaceae) from Australia, India, and Thailand".Botanical Studies 61.1(2020). |
入库方式： OAI收割
来源：武汉植物园
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