SMRT and Illumina RNA sequencing reveal novel insights into the heat stress response and crosstalk with leaf senescence in tall fescue
文献类型：期刊论文
| 作者 | Qian,Yiguang1; Cao,Liwen2; Zhang,Qiang2; Amee,Maurice2; Chen,Ke3; Chen,Liang2,4 |
| 刊名 | BMC Plant Biology
 |
| 出版日期 | 2020-08-03 |
| 卷号 | 20期号:1 |
| 关键词 | Tall fescue Short- and long-term heat stress Senescence SMRT and Illumina RNA-Seq Responsive genes Alternative splicing |
| DOI | 10.1186/s12870-020-02572-4 |
| 英文摘要 | AbstractBackgroundAs a cool-season grass species, tall fescue (Festuca arundinacea) is challenged by increasing temperatures. Heat acclimation or activation of leaf senescence, are two main strategies when tall fescue is exposed to heat stress (HS). However, lacking a genome sequence, the complexity of hexaploidy nature, and the short read of second-generation sequencing hinder a comprehensive understanding of the mechanism. This study aims to characterize the molecular mechanism of heat adaptation and heat-induced senescence at transcriptional and post-transcriptional levels.ResultsTranscriptome of heat-treated (1?h and 72?h) and senescent leaves of tall fescue were generated by combining single-molecular real-time and Illumina sequencing. In total, 4076; 6917, and 11,918 differentially expressed genes (DEGs) were induced by short- and long-term heat stress (HS), and senescence, respectively. Venn and bioinformatics analyses of DEGs showed that short-term HS strongly activated heat shock proteins (Hsps) and heat shock factors (Hsfs), as well as specifically activated FK506-binding proteins (FKBPs), calcium signaling genes, glutathione S-transferase genes, photosynthesis-related genes, and phytohormone signaling genes. By contrast, long-term HS shared most of DEGs with senescence, including the up-regulated chlorophyll catabolic genes, phytohormone synthesis/degradation genes, stress-related genes, and NACs, and the down-regulated photosynthesis-related genes, FKBPs, and catalases. Subsequently, transient overexpression in tobacco showed that FaHsfA2a (up-regulated specifically by short-term HS) reduced cell membrane damages caused by HS, but FaNAC029 and FaNAM-B1 (up-regulated by long-term HS and senescence) increased the damages. Besides, alternative splicing was widely observed in HS and senescence responsive genes, including Hsps, Hsfs, and phytohormone signaling/synthesis genes.ConclusionsThe short-term HS can stimulate gene responses and improve thermotolerance, but long-term HS is a damage and may accelerate leaf senescence. These results contribute to our understanding of the molecular mechanism underlying heat adaptation and heat-induced senescence. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/S12870-020-02572-4 |
| 出版者 | BioMed Central |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14373]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chen,Ke; Chen,Liang |
| 作者单位 | 1.Shenzhen University; Shenzhen Key Laboratory of Environmental Chemistry and Ecological Remediation, College of Chemistry and Environmental Engineering 2.CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences 3.South-Central University for Nationalities; College of Resources and Environmental Science, Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Hubei Key Laboratory of Catalysis and Materials Science 4.Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences |
| 推荐引用方式 GB/T 7714 | Qian,Yiguang,Cao,Liwen,Zhang,Qiang,et al. SMRT and Illumina RNA sequencing reveal novel insights into the heat stress response and crosstalk with leaf senescence in tall fescue[J]. BMC Plant Biology,2020,20(1). |
| APA | Qian,Yiguang,Cao,Liwen,Zhang,Qiang,Amee,Maurice,Chen,Ke,&Chen,Liang.(2020).SMRT and Illumina RNA sequencing reveal novel insights into the heat stress response and crosstalk with leaf senescence in tall fescue.BMC Plant Biology,20(1). |
| MLA | Qian,Yiguang,et al."SMRT and Illumina RNA sequencing reveal novel insights into the heat stress response and crosstalk with leaf senescence in tall fescue".BMC Plant Biology 20.1(2020). |
入库方式： OAI收割
来源：武汉植物园
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