Comparative transcriptome combined with metabolome analyses revealed key factors involved in nitric oxide (NO)-regulated cadmium stress adaptation in tall fescue
文献类型：期刊论文
| 作者 | Zhu,Huihui1,2; Ai,Honglian3; Hu,Zhengrong1; Du,Dongyun2; Sun,Jie2; Chen,Ke2; Chen,Liang1,4 |
| 刊名 | BMC Genomics
 |
| 出版日期 | 2020-08-31 |
| 卷号 | 21期号:1 |
| 关键词 | Tall fescue Cd stress Nitric oxide RNA-Seq Metabolite profiling Detoxification mechanism |
| DOI | 10.1186/s12864-020-07017-8 |
| 英文摘要 | AbstractBackgroundIt has been reported that nitric oxide (NO) could ameliorate cadmium (Cd) toxicity in tall fescue; however, the underlying mechanisms of NO mediated Cd detoxification are largely unknown. In this study, we investigated the possible molecular mechanisms of Cd detoxification process by comparative transcriptomic and metabolomic approaches.ResultsThe application of Sodium nitroprusside (SNP) as NO donor decreased the Cd content of tall fescue by 11% under Cd stress (T1 treatment), but the Cd content was increased by 24% when treated with Carboxy-PTIO (c-PTIO) together with Nitro-L-arginine methyl ester (L-NAME) (T2 treatment). RNA-seq analysis revealed that 904 (414 up- and 490 down-regulated) and 118 (74 up- and 44 down-regulated) DEGs were identified in the T1 vs Cd (only Cd treatment) and T2 vs Cd comparisons, respectively. Moreover, metabolite profile analysis showed that 99 (65 up- and 34-down- regulated) and 131 (45 up- and 86 down-regulated) metabolites were altered in the T1 vs Cd and T2 vs Cd comparisons, respectively. The integrated analyses of transcriptomic and metabolic data showed that 81 DEGs and 15 differentially expressed metabolites were involved in 20 NO-induced pathways. The dominant pathways were antioxidant activities such as glutathione metabolism, arginine and proline metabolism, secondary metabolites such as flavone and flavonol biosynthesis and phenylpropanoid biosynthesis, ABC transporters, and nitrogen metabolism.ConclusionsIn general, the results revealed that there are three major mechanisms involved in NO-mediated Cd detoxification in tall fescue, including (a) antioxidant capacity enhancement; (b) accumulation of secondary metabolites related to cadmium chelation and sequestration; and (c) regulation of cadmium ion transportation, such as ABC transporter activation. In conclusion, this study provides new insights into the NO-mediated cadmium stress response. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/S12864-020-07017-8 |
| 出版者 | BioMed Central |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14381]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chen,Ke; Chen,Liang |
| 作者单位 | 1.Chinese Academy of Sciences; CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden 2.South-Central University for Nationalities; College of Resources and Environmental Science 3.South-Central University for Nationalities; College of Pharmacy 4.Chinese Academy of Sciences; Center of Economic Botany, Core Botanical Gardens |
| 推荐引用方式 GB/T 7714 | Zhu,Huihui,Ai,Honglian,Hu,Zhengrong,et al. Comparative transcriptome combined with metabolome analyses revealed key factors involved in nitric oxide (NO)-regulated cadmium stress adaptation in tall fescue[J]. BMC Genomics,2020,21(1). |
| APA | Zhu,Huihui.,Ai,Honglian.,Hu,Zhengrong.,Du,Dongyun.,Sun,Jie.,...&Chen,Liang.(2020).Comparative transcriptome combined with metabolome analyses revealed key factors involved in nitric oxide (NO)-regulated cadmium stress adaptation in tall fescue.BMC Genomics,21(1). |
| MLA | Zhu,Huihui,et al."Comparative transcriptome combined with metabolome analyses revealed key factors involved in nitric oxide (NO)-regulated cadmium stress adaptation in tall fescue".BMC Genomics 21.1(2020). |
入库方式： OAI收割
来源：武汉植物园
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