Light- and Temperature-Induced Expression of an R2R3-MYB Gene Regulates Anthocyanin Biosynthesis in Red-Fleshed Kiwifruit
文献类型：期刊论文
| 作者 | Yu, Min1,2; Man, Yuping2; Wang, Yanchang2 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2019-10-02 |
| 卷号 | 20期号:20页码:22 |
| 关键词 | AcMYB10 kiwifruit R2R3-MYB anthocyanin light temperature |
| DOI | 10.3390/ijms20205228 |
| 英文摘要 | The R2R3 MYB genes associated with the flavonoid/anthocyanidin pathway feature two repeats, and represent the most abundant classes of MYB genes in plants; however, the physiological role and regulatory function of most R2R3 MYBs remain poorly understood in kiwifruit (Actinidia). Here, genome-wide analysis identified 155 R2R3-MYBs in the `Red 50 version of the Actinidia chinensis genome. Out of 36 anthocyanin-related AccR2R3-MYBs, AcMYB10 was the most highly expressed in inner pericarp of red-fleshed kiwifruit. The expression of AcMYB10 was highly correlated with anthocyanin accumulation in natural pigmentation during fruit ripening and light-/temperature-induced pigmentation in the callus. AcMYB10 is localized in the nuclei and has transcriptional activation activity. Overexpression of AcMYB10 elevates anthocyanin accumulation in transgenic A. chinensis. In comparison, A. chinensis fruit infiltrated with virus-induced gene silencing showed delayed red coloration, lower anthocyanin content, and lower expression of AcMYB10. The transient expression experiment in Nicotiana tabacum leaves and Actinidia arguta fruit indicated the interaction of AcMYB10 with AcbHLH42 might strongly activate anthocyanin biosynthesis by activating the transcription of AcLDOX and AcF3GT. In conclusion, this study provides novel molecular information about R2R3-MYBs in kiwifruit, advances our understanding of light- and temperature-induced anthocyanin accumulation, and demonstrates the important function of AcMYB10 in the biosynthesis of anthocyanin in kiwifruit. |
| 资助项目 | Natural Science Foundation of China (NSFC)[31501745] ; Natural Science Foundation of China (NSFC)[30671433] ; Natural Science Foundation of China (NSFC)[31171945] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000498822800260 |
| 出版者 | MDPI |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14437]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wang, Yanchang |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Min,Man, Yuping,Wang, Yanchang. Light- and Temperature-Induced Expression of an R2R3-MYB Gene Regulates Anthocyanin Biosynthesis in Red-Fleshed Kiwifruit[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(20):22. |
| APA | Yu, Min,Man, Yuping,&Wang, Yanchang.(2019).Light- and Temperature-Induced Expression of an R2R3-MYB Gene Regulates Anthocyanin Biosynthesis in Red-Fleshed Kiwifruit.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(20),22. |
| MLA | Yu, Min,et al."Light- and Temperature-Induced Expression of an R2R3-MYB Gene Regulates Anthocyanin Biosynthesis in Red-Fleshed Kiwifruit".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.20(2019):22. |
入库方式： OAI收割
来源：武汉植物园
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