Intra-individual heteroplasmy in the Gentiana tongolensis plastid genome (Gentianaceae)
文献类型：期刊论文
| 作者 | Sun, Shan-Shan1; Zhou, Xiao-Jun1; Li, Zhi-Zhong2,3; Song, Hong-Yang1; Long, Zhi-Cheng4; Fu, Peng-Cheng1 |
| 刊名 | PEERJ
 |
| 出版日期 | 2019-11-27 |
| 卷号 | 7页码:15 |
| 关键词 | Gentiana tongolensis SNP Plastid genome Heteroplasmy |
| ISSN号 | 2167-8359 |
| DOI | 10.7717/peerj.8025 |
| 英文摘要 | Chloroplasts are typically inherited from the female parent and are haploid in most angiosperms, but rare intra-individual heteroplasmy in plastid genomes has been reported in plants. Here, we report an example of plastome heteroplasmy and its characteristics in Gentiana tongolensis (Gentianaceae). The plastid genome of G. tongolensis is 145,757 bp in size and is missing parts of petD gene when compared with other Gentiana species. A total of 112 single nucleotide polymorphisms (SNPs) and 31 indels with frequencies of more than 2% were detected in the plastid genome, and most were located in protein coding regions. Most sites with SNP frequencies of more than 10% were located in six genes in the LSC region. After verification via cloning and Sanger sequencing at three loci, heteroplasmy was identified in different individuals. The cause of heteroplasmy at the nucleotide level in plastome of G. tongolensis is unclear from the present data, although biparental plastid inheritance and transfer of plastid DNA seem to be most likely. This study implies that botanists should reconsider the heredity and evolution of chloroplasts and be cautious with using chloroplasts as genetic markers, especially in Gentiana. |
| 资助项目 | National Natural Science Foundation of China[31600296] ; National Natural Science Foundation of China[31870697] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000498840700003 |
| 出版者 | PEERJ INC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14572]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhou, Xiao-Jun; Fu, Peng-Cheng |
| 作者单位 | 1.Luoyang Normal Univ, Coll Life Sci, Luoyang, Henan, Peoples R China 2.Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, Wuhan, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.HostGene Co Ltd, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Shan-Shan,Zhou, Xiao-Jun,Li, Zhi-Zhong,et al. Intra-individual heteroplasmy in the Gentiana tongolensis plastid genome (Gentianaceae)[J]. PEERJ,2019,7:15. |
| APA | Sun, Shan-Shan,Zhou, Xiao-Jun,Li, Zhi-Zhong,Song, Hong-Yang,Long, Zhi-Cheng,&Fu, Peng-Cheng.(2019).Intra-individual heteroplasmy in the Gentiana tongolensis plastid genome (Gentianaceae).PEERJ,7,15. |
| MLA | Sun, Shan-Shan,et al."Intra-individual heteroplasmy in the Gentiana tongolensis plastid genome (Gentianaceae)".PEERJ 7(2019):15. |
入库方式： OAI收割
来源：武汉植物园
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