The pH of the leaf apoplast is critical for the formation of Pseudomonas syringae-induced lesions on leaves of the common bean (Phaseolus vulgaris)
文献类型：期刊论文
| 作者 | Geilfus, Christoph-Martin1; Wang, Li2; Wu, Jiawen3; Xue, Cheng4 |
| 刊名 | PLANT SCIENCE
 |
| 出版日期 | 2020 |
| 卷号 | 290页码:7 |
| 关键词 | Apoplast Alkalinization Sucrose Auxin Phaseolus Vulgaris Pseudomonas Syringae |
| ISSN号 | 0168-9452 |
| DOI | 10.1016/j.plantsci.2019.110328 |
| 英文摘要 | Inoculating a virulent strain of Pseudomonas syringae pv. phaseolicola (Pph) into the leaf of common bean (Phaseolus vulgaris) causes the leaf apoplast to alkalinize. Whether or not this apoplastic pH event facilitates virulence of Pph in interaction with common bean is unclear. For elucidating this topic, (i) Pph colonization of the common bean leaf apoplast, (ii) the formation of bacterial lesions, and (iii) apoplastic sucrose concentration were investigated in relation to the apoplastic leaf pH. For this, the Pph-induced leaf apoplastic alkalinization was attenuated by spray application of either a synthetic auxin or an acidic pH buffer. Apoplastic pH was quantified in planta via microscopy-based pH imaging. Apoplastic washing fluids were extracted to quantify both colonization of bacteria in leaf apoplast and the concentration of apoplastic sucrose. Results reveal that the apoplastic alkalinization facilitated bacterial colonization of the apoplast. Number of colony forming units and area of bacterial lesions were reduced when Pph-induced apoplastic alkalinization was attenuated by foliar application of a synthetic auxin or acidic pH buffer. Application of both agents attenuated the Pph-induced increase of sucrose in the leaf apoplast, which is nutrient for bacteria. Data demonstrate that the Pph-mediated leaf apoplastic alkalinisation favours bacterial colonization. |
| 资助项目 | DFG[GE 3111/1-1] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000501940100031 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14606]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Geilfus, Christoph-Martin; Wang, Li |
| 作者单位 | 1.Humboldt Univ, Albrecht Daniel Thaer Inst Agr & Hort Sci, Fac Life Sci, Div Controlled Environm Hort, Albrecht Thaer Weg 1, D-14195 Berlin, Germany 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Aquat Bot & Watershed Ecol, Wuhan 430074, Hubei, Peoples R China 3.Yanan Univ, Coll Life Sci, Yanan 716000, Shaanxi, Peoples R China 4.Agr Univ Hebei, China Coll Resources & Environm Sci, Baoding, Hebei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geilfus, Christoph-Martin,Wang, Li,Wu, Jiawen,et al. The pH of the leaf apoplast is critical for the formation of Pseudomonas syringae-induced lesions on leaves of the common bean (Phaseolus vulgaris)[J]. PLANT SCIENCE,2020,290:7. |
| APA | Geilfus, Christoph-Martin,Wang, Li,Wu, Jiawen,&Xue, Cheng.(2020).The pH of the leaf apoplast is critical for the formation of Pseudomonas syringae-induced lesions on leaves of the common bean (Phaseolus vulgaris).PLANT SCIENCE,290,7. |
| MLA | Geilfus, Christoph-Martin,et al."The pH of the leaf apoplast is critical for the formation of Pseudomonas syringae-induced lesions on leaves of the common bean (Phaseolus vulgaris)".PLANT SCIENCE 290(2020):7. |
入库方式： OAI收割
来源：武汉植物园
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