Quantitative ubiquitylomics approach for characterizing the dynamic change and extensive modulation of ubiquitylation in rice seed germination
文献类型：期刊论文
| 作者 | He, Dongli1,2; Li, Ming1,2; Damaris, Rebecca N.1; Bu, Chen3; Xue, Jianyou3; Yang, Pingfang1 |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2019-12-11 |
| 页码 | 18 |
| 关键词 | lysine ubiquitylation quantitative ubiquitylome embryo seed germination rice |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.14593 |
| 英文摘要 | During seed germination, cells embark on extensive post-transcriptional and post-translational modifications (PTM), providing a perfect platform to study these events in embryo rebooting from relative quiescenct to highly active state. PR-619, a deubiquitylase inhibitor, delayed the rice seed germination and resulted in the accumulation of ubiquitylated proteins, which indicated the protein ubiquitylation is involved in this process. Using the K--GG antibody enrichment method integrated with high-resolution mass spectrometry, a list of 2576 lysine ubiquitylated (Kub) sites in 1171 proteins was compiled for rice embryos at 0, 12 and 24 h after imbibition (HAI). Of these, the abundance of 1419 Kub sites in 777 proteins changed significantly. Most of them substantially increased within the first 12 HAI, which is similar to the dynamic state previously observed for protein phosphorylation, implying that the first 12 HAI are essential for subsequent switch during rice seed germination. We also quantitatively analyzed the embryo proteome in these samples. Generally, a specific protein's abundance in the ubiquitylome was uncorrelated to that in the proteome. The differentially ubiquitinated proteins were greatly enriched in the categories of protein processing, DNA and RNA processing/regulation related, signaling, and transport. The DiGly footprint of the Kub sites was significantly reduced on K48, a linkage typically associated with proteasome-mediated degradation. These observations suggest ubiquitylation may modulate the protein function more than providing 26S degradation signals in the early stage of rice seed germination. Revealing this comprehensive ubiquitylome greatly increases our understanding of this critical PTM during seed germination. |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000502080200001 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14613]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yang, Pingfang |
| 作者单位 | 1.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Hubei, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Germ Plasm Enhancement & Special Ag, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 3.Jingjie PTM BioLab Hangzhou Co Ltd, Hangzhou 310018, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Dongli,Li, Ming,Damaris, Rebecca N.,et al. Quantitative ubiquitylomics approach for characterizing the dynamic change and extensive modulation of ubiquitylation in rice seed germination[J]. PLANT JOURNAL,2019:18. |
| APA | He, Dongli,Li, Ming,Damaris, Rebecca N.,Bu, Chen,Xue, Jianyou,&Yang, Pingfang.(2019).Quantitative ubiquitylomics approach for characterizing the dynamic change and extensive modulation of ubiquitylation in rice seed germination.PLANT JOURNAL,18. |
| MLA | He, Dongli,et al."Quantitative ubiquitylomics approach for characterizing the dynamic change and extensive modulation of ubiquitylation in rice seed germination".PLANT JOURNAL (2019):18. |
入库方式： OAI收割
来源：武汉植物园
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