Transcriptomic Analysis of Dark-Induced Senescence in Bermudagrass (Cynodon dactylon)
文献类型：期刊论文
| 作者 | Fan, Jibiao2,3,4; Lou, Yanhong1; Shi, Haiyan5; Chen, Liang3,4; Cao, Liwen3,4 |
| 刊名 | PLANTS-BASEL
 |
| 出版日期 | 2019-12-01 |
| 卷号 | 8期号:12页码:17 |
| 关键词 | transcriptome senescence darkness bermudagrass |
| DOI | 10.3390/plants8120614 |
| 英文摘要 | Leaf senescence induced by prolonged light deficiency is inevitable whenever turfgrass is cultivated in forests, and this negatively influences the survival and aesthetic quality of the turfgrass. However, the mechanism underlying dark-induced senescence in turfgrass remained obscure. In this study, RNA sequencing was performed to analyze how genes were regulated in response to dark-induced leaf senescence in bermudagrass. A total of 159,207 unigenes were obtained with a mean length of 948 bp. The differential expression analysis showed that a total of 59,062 genes, including 52,382 up-regulated genes and 6680 down-regulated genes were found to be differentially expressed between control leaves and senescent leaves induced by darkness. Subsequent bioinformatics analysis showed that these differentially expressed genes (DEGs) were mainly related to plant hormone (ethylene, abscisic acid, jasmonic acid, auxin, cytokinin, gibberellin, and brassinosteroid) signal transduction, N-glycan biosynthesis, and protein processing in the endoplasmic reticulum. In addition, transcription factors, such as WRKY, NAC, HSF, and bHLH families were also responsive to dark-induced leaf senescence in bermudagrass. Finally, qRT-PCR analysis of six randomly selected DEGs validated the accuracy of sequencing results. Taken together, our results provide basic information of how genes respond to darkness, and contribute to the understanding of comprehensive mechanisms of dark-induced leaf senescence in turfgrass. |
| 资助项目 | National Natural Science Foundation of China[31702165] ; National Natural Science Foundation of China[31672482] ; China Postdoctoral Science Foundation[2017M621838] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000506648700088 |
| 出版者 | MDPI |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14721]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chen, Liang; Cao, Liwen |
| 作者单位 | 1.Shandong Agr Univ, Coll Resources & Environm, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Daizong Rd, Tai An 271018, Shandong, Peoples R China 2.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China 3.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan 430074, Peoples R China 4.Chinese Acad Sci, Core Bot Gardens, Ctr Econ Bot, Wuhan 430074, Peoples R China 5.Agr Univ Hebei, Coll Hort, Baoding 071001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Jibiao,Lou, Yanhong,Shi, Haiyan,et al. Transcriptomic Analysis of Dark-Induced Senescence in Bermudagrass (Cynodon dactylon)[J]. PLANTS-BASEL,2019,8(12):17. |
| APA | Fan, Jibiao,Lou, Yanhong,Shi, Haiyan,Chen, Liang,&Cao, Liwen.(2019).Transcriptomic Analysis of Dark-Induced Senescence in Bermudagrass (Cynodon dactylon).PLANTS-BASEL,8(12),17. |
| MLA | Fan, Jibiao,et al."Transcriptomic Analysis of Dark-Induced Senescence in Bermudagrass (Cynodon dactylon)".PLANTS-BASEL 8.12(2019):17. |
入库方式： OAI收割
来源：武汉植物园
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