Ectopic expression of Medicago truncatula homeodomain finger protein, MtPHD6, enhances drought tolerance in Arabidopsis
文献类型：期刊论文
| 作者 | Quan, Wenli2; Liu, Xun3; Wang, Lihua2; Yin, Mingzhu4; Yang, Li5,6,7; Chan, Zhulong1,5,6,7 |
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2019-12-16 |
| 卷号 | 20期号:1页码:16 |
| ISSN号 | 1471-2164 |
| 关键词 | ABA Drought stress Interaction network Medicago truncatula PHD finger protein Zinc finger protein |
| DOI | 10.1186/s12864-019-6350-5 |
| 英文摘要 | Background: The plant homeodomain (PHD) finger is a Cys(4)HisCys(3)-type zinc finger which promotes protein-protein interactions and binds to the cis-acting elements in the promoter regions of target genes. In Medicago truncatula, five PHD homologues with full-length sequence were identified. However, the detailed function of PHD genes was not fully addressed. Results: In this study, we characterized the function of MtPHD6 during plant responses to drought stress. MtPHD6 was highly induced by drought stress. Ectopic expression of MtPHD6 in Arabidopsis enhanced tolerance to osmotic and drought stresses. MtPHD6 transgenic plants exhibited decreased water loss rate, MDA and ROS contents, and increased leaf water content and antioxidant enzyme activities under drought condition. Global transcriptomic analysis revealed that MtPHD6 reprogramed transcriptional networks in transgenic plants. Expression levels of ABA receptor PYR/PYLs, ZINC FINGER, AP2/EREBP and WRKY transcription factors were mainly up-regulated after transformation of MtPHD6. Interaction network analysis showed that ZINC FINGER, AP2/EREBP and WRKY interacted with each other and downstream stress induced proteins. Conclusions: We proposed that ZINC FINGER, AP2/EREBP and WRKY transcription factors were activated through ABA dependent and independent pathways to increase drought tolerance of MtPHD6 transgenic plants. |
| 资助项目 | National Key Research and Development Program of China[2017YFD0201305] ; Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation[2016RC010] ; National Natural Science Foundation of China[31872143] ; Youth Project of Science and Technology Department of Hubei Education Department[Q20182702] ; China Scholarship Council |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| 出版者 | BMC |
| WOS记录号 | WOS:000508020400002 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14769]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chan, Zhulong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Wuhan, Hubei, Peoples R China 2.Hubei Engn Univ, Coll Life Sci & Technol, Key Lab Qual Control Characterist Fruits & Vegeta, Xiaogan, Hubei, Peoples R China 3.Univ Bonn, Inst Mol Physiol & Biotechnol Plants IMBIO, Bonn, Germany 4.Hubei Univ Chinese Med, Coll Basic Med, Wuhan, Hubei, Peoples R China 5.Minist Educ, Key Lab Hort Plant Biol, Wuhan, Hubei, Peoples R China 6.Minist Agr, Key Lab Urban Agr Cent China, Wuhan, Hubei, Peoples R China 7.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Quan, Wenli,Liu, Xun,Wang, Lihua,et al. Ectopic expression of Medicago truncatula homeodomain finger protein, MtPHD6, enhances drought tolerance in Arabidopsis[J]. BMC GENOMICS,2019,20(1):16. |
| APA | Quan, Wenli,Liu, Xun,Wang, Lihua,Yin, Mingzhu,Yang, Li,&Chan, Zhulong.(2019).Ectopic expression of Medicago truncatula homeodomain finger protein, MtPHD6, enhances drought tolerance in Arabidopsis.BMC GENOMICS,20(1),16. |
| MLA | Quan, Wenli,et al."Ectopic expression of Medicago truncatula homeodomain finger protein, MtPHD6, enhances drought tolerance in Arabidopsis".BMC GENOMICS 20.1(2019):16. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。