Advances in MS Based Strategies for Probing Ligand-Target Interactions: Focus on Soft Ionization Mass Spectrometric Techniques
文献类型：期刊论文
| 作者 | Chen, Guilin2,3; Fan, Minxia2,3,4; Liu, Ye2,3; Sun, Baoqing1; Liu, Meixian5; Wu, Jianlin5; Li, Na5; Guo, Mingquan2,3 |
| 刊名 | FRONTIERS IN CHEMISTRY
 |
| 出版日期 | 2019-10-23 |
| 卷号 | 7页码:17 |
| 关键词 | ligand-target interactions mass spectrometry soft ionization drug discovery ESI-MS MALDI-MS |
| ISSN号 | 2296-2646 |
| DOI | 10.3389/fchem.2019.00703 |
| 英文摘要 | The non-covalent interactions between small drug molecules and disease-related proteins (ligand-target interactions) mediate various pharmacological processes in the treatment of different diseases. The development of the analytical methods to assess those interactions, including binding sites, binding energies, stoichiometry and association-dissociation constants, could assist in clarifying the mechanisms of action, precise treatment of targeted diseases as well as the targeted drug discovery. For the last decades, mass spectrometry (MS) has been recognized as a powerful tool to study the non-covalent interactions of the ligand-target complexes with the characteristics of high sensitivity, high-resolution, and high-throughput. Soft ionization mass spectrometry, especially the electrospray mass spectrometry (ESI-MS) and matrix assisted laser desorption ionization mass spectrometry (MALDI-MS), could achieve the complete transformation of the target analytes into the gas phase, and subsequent detection of the small drug molecules and disease-related protein complexes, and has exerted great advantages for studying the drug ligands-protein targets interactions, even in case of identifying active components as drug ligands from crude extracts of medicinal plants. Despite of other analytical techniques for this purpose, such as the NMR and X-ray crystallography, this review highlights the principles, research hotspots and recent applications of the soft ionization mass spectrometry and its hyphenated techniques, including hydrogen-deuterium exchange mass spectrometry (HDX-MS), chemical cross-linking mass spectrometry (CX-MS), and ion mobility spectrometry mass spectrometry (IMS-MS), in the study of the non-covalent interactions between small drug molecules and disease-related proteins. |
| 资助项目 | National Natural Science Foundation of China[81673580] ; National Natural Science Foundation of China[81903791] ; Major Project for Special Technology Innovation of Hubei Province[2017AHB054] ; Macao Science and Technology Development Fund[0090/2018/A3] ; Natural Science Foundation of Hubei Province[2019CFB254] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000494675900001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14854]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Mingquan |
| 作者单位 | 1.Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Dis, Natl Clin Ctr Resp Dis,State Key Lab Resp Dis, Guangzhou, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Hubei, Peoples R China 3.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan, Hubei, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 5.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa, Macao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Guilin,Fan, Minxia,Liu, Ye,et al. Advances in MS Based Strategies for Probing Ligand-Target Interactions: Focus on Soft Ionization Mass Spectrometric Techniques[J]. FRONTIERS IN CHEMISTRY,2019,7:17. |
| APA | Chen, Guilin.,Fan, Minxia.,Liu, Ye.,Sun, Baoqing.,Liu, Meixian.,...&Guo, Mingquan.(2019).Advances in MS Based Strategies for Probing Ligand-Target Interactions: Focus on Soft Ionization Mass Spectrometric Techniques.FRONTIERS IN CHEMISTRY,7,17. |
| MLA | Chen, Guilin,et al."Advances in MS Based Strategies for Probing Ligand-Target Interactions: Focus on Soft Ionization Mass Spectrometric Techniques".FRONTIERS IN CHEMISTRY 7(2019):17. |
入库方式： OAI收割
来源：武汉植物园
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