Genome-wide analysis of coding and non-coding RNA reveals a conserved miR164-NAC regulatory pathway for fruit ripening
文献类型：期刊论文
| 作者 | Wang, Wen-qiu2,3; Wang, Jian2; Wu, Ying-ying2; Li, Da-wei4; Allan, Andrew C.1,5; Yin, Xue-ren2,3 |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2019-11-11 |
| 页码 | 17 |
| 关键词 | aroma cell wall degradome ethylene fruit ripening miR164 NAC transcriptome |
| ISSN号 | 0028-646X |
| DOI | 10.1111/nph.16233 |
| 英文摘要 | Kiwifruit (Actinidia spp.) is a climacteric fruit with high sensitivity to ethylene, influenced by multiple ethylene-responsive structural genes and transcription factors. However, the roles of other post-transcriptional regulators (e.g. miRNAs) necessary for ripening remain elusive. High-throughput sequencing sRNAome, degradome and transcriptome methods were used to identify further contributors to ripening control in the kiwifruit (A. deliciosa cv 'Hayward'). Two NAM/ATAF/CUC domain transcription factors (AdNAC6 and AdNAC7), both predicted targets for miR164, showed significant upregulation by exogenous ethylene. Gene expression analysis and luciferase reporter assays indicated that Ade-miR164 and one of its precursor miRNAs (Ade-MIR164b) were repressed by ethylene treatment and negatively correlated with AdNAC6/7 expression. Subsequent analysis indicated that both AdNAC6 and AdNAC7 proteins are transcriptional activators and physically bind the promoters of AdACS1 (1-aminocyclopropane-1-carboxylate synthase), AdACO1 (1-aminocyclopropane-1-carboxylic acid oxidase), AdMAN1 (endo-beta-mannanase) and AaTPS1 (terpene synthase). Moreover, subcellular analysis indicated that the location of the AdNAC6/7 proteins was influenced by Ade-miR164. Multiple omics-based approaches revealed a novel regulatory link for fruit ripening that involved ethylene-miR164-NAC. The regulatory pathway for miR164-NAC is present in various fruit (e.g. Rosaceae fruit, citrus, grape), with implications for fruit ripening regulation. |
| 资助项目 | National Key Research and Development Program[2018YFD1000200] ; National Natural Science Foundation of China[31722042] ; Natural Science Foundation of Zhejiang Province, China[LR16C150001] ; Fok Ying Tung Education Foundation, China[161028] ; 111 Project[B17039] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000495449500001 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14870]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Allan, Andrew C.; Yin, Xue-ren |
| 作者单位 | 1.Univ Auckland, Sch Biol Sci, Private Bag 92019, Auckland, New Zealand 2.Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China 3.Zhejiang Univ, State Agr Minist Lab Hort Plant Growth Dev & Qual, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China 4.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 5.New Zealand Inst Plant & Food Res Ltd, Private Bag 92169, Auckland, New Zealand |
| 推荐引用方式 GB/T 7714 | Wang, Wen-qiu,Wang, Jian,Wu, Ying-ying,et al. Genome-wide analysis of coding and non-coding RNA reveals a conserved miR164-NAC regulatory pathway for fruit ripening[J]. NEW PHYTOLOGIST,2019:17. |
| APA | Wang, Wen-qiu,Wang, Jian,Wu, Ying-ying,Li, Da-wei,Allan, Andrew C.,&Yin, Xue-ren.(2019).Genome-wide analysis of coding and non-coding RNA reveals a conserved miR164-NAC regulatory pathway for fruit ripening.NEW PHYTOLOGIST,17. |
| MLA | Wang, Wen-qiu,et al."Genome-wide analysis of coding and non-coding RNA reveals a conserved miR164-NAC regulatory pathway for fruit ripening".NEW PHYTOLOGIST (2019):17. |
入库方式： OAI收割
来源：武汉植物园
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