Characterization of covalent protein modification by triclosan in vivo and in vitro via three-dimensional liquid chromatography-mass spectrometry: New insight into its adverse effects
文献类型：期刊论文
| 作者 | Liu, Meixian2; Li, Na2; Zhang, Yida2; Zheng, Zhiyuan2; Zhuo, Yue2; Sun, Baoqing3; Bai, Li-Ping2; Zhang, Mingming4; Guo, Ming-Quan1; Wu, Jian-Lin2 |
| 刊名 | ENVIRONMENT INTERNATIONAL
 |
| 出版日期 | 2020-03-01 |
| 卷号 | 136页码:10 |
| 关键词 | Triclosan Adverse effects Reactive metabolites Covalent protein modification In vivo Three-dimensional-liquid chromatography-mass spectrometry |
| ISSN号 | 0160-4120 |
| DOI | 10.1016/j.envint.2019.105423 |
| 英文摘要 | Triclosan (TCS), an antimicrobial agent widely used in personal care products and ubiquitously exists in environment, has drawn increasing concern due to its potential to exert multiple adverse effects, ranging from endocrine disruption to carcinogenesis. However, the mechanism of these adverse effects is still not fully elucidated. More and more studies have shown that chemical reactive metabolites (RMs) covalently binding to proteins is a possible reason for these adverse effects, but there is still a lack of appropriate methods to predict or evaluate these adverse effects due to the extremely low abundance of the modified proteins in complex biological samples. In this study, we attempted to address this problem and investigate the possible mechanism of TCS adverse effects by a shotgun proteomics approach based on three-dimensional-liquid chromatography-mass spectrometry (3D-LC-MS). First, the in vitro incubation with model amino acids and protein in microsomes showed that TCS could react with cysteine residue of proteins through 3 types of RMs. Then, a 3D-LC-MS approach was developed to sensitively determine the low abundant modified proteins, which resulted in the identification of 45 TCS-modified proteins, including albumin, haptoglobin and NR1I2, in rats. STRING analysis indicated that these modified proteins mainly were involved in reproductive and development system, endocrine and immune system, and carcinogenesis, which were in accord with the main reported TCS-induced adverse effects and suggested that the covalent modification of TCS RMs for proteins might affect their activities and functions, thus inducing serious adverse effects. This study provided a new insight into the mechanism of TCS adverse effects and may serve as a valuable method to predict or evaluate adverse effects of ubiquitous chemicals. |
| 资助项目 | Science and Technology Development Fund, Macau SAR[009/2017/A1] ; Science and Technology Development Fund, Macau SAR[0090/2018/A3] ; Open Project of State Key Laboratory of Respiratory Disease[SKLRDOP-201803] ; National Natural Science Foundation of China[81673580] |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000512533700014 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14886]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Li, Na; Wu, Jian-Lin |
| 作者单位 | 1.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 2.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Ave Wai Long, Taipa, Macau, Peoples R China 3.Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Dis, State Key Lab Resp Dis,Natl Clin Ctr Resp Dis, Guangzhou, Guangdong, Peoples R China 4.Nanjing Univ, Med Sch, Drum Tower Hosp, Dept Gastroenterol, Nanjing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Meixian,Li, Na,Zhang, Yida,et al. Characterization of covalent protein modification by triclosan in vivo and in vitro via three-dimensional liquid chromatography-mass spectrometry: New insight into its adverse effects[J]. ENVIRONMENT INTERNATIONAL,2020,136:10. |
| APA | Liu, Meixian.,Li, Na.,Zhang, Yida.,Zheng, Zhiyuan.,Zhuo, Yue.,...&Wu, Jian-Lin.(2020).Characterization of covalent protein modification by triclosan in vivo and in vitro via three-dimensional liquid chromatography-mass spectrometry: New insight into its adverse effects.ENVIRONMENT INTERNATIONAL,136,10. |
| MLA | Liu, Meixian,et al."Characterization of covalent protein modification by triclosan in vivo and in vitro via three-dimensional liquid chromatography-mass spectrometry: New insight into its adverse effects".ENVIRONMENT INTERNATIONAL 136(2020):10. |
入库方式： OAI收割
来源：武汉植物园
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