Genome-wide analysis of maize MBD gene family and expression profiling under abiotic stress treatment at the seedling stage
文献类型：期刊论文
| 作者 | Qian, Yexiong1; Ren, Qiaoyu1; Jiang, Lingyu1; Zhang, Jing1; Chen, Changle1; Chen, Liang2 |
| 刊名 | PLANT BIOTECHNOLOGY REPORTS
 |
| 出版日期 | 2020-02-25 |
| 页码 | 16 |
| 关键词 | Epigenetics MBD protein Trans-acting factor Gene expression Zea mays L |
| ISSN号 | 1863-5466 |
| DOI | 10.1007/s11816-020-00607-8 |
| 英文摘要 | Methyl-CpG-binding domain (MBD) proteins in plants are important trans-acting factors specifically recognizing methylated DNA. The MBD proteins can compact chromatins to repress transcription by recruiting chromatin-modifying complexes that contain histone deacetylase activities and chromatin remodeling factors, and play a crucial biological role in the growth and development in plants. Currently, very little is known regarding the structure and function of MBD genes in plants. In this study, we performed a genome-wide identification and expression profile analysis of maize MBD genes (ZmMBDs) from the latest version of the maize (B73) genome. By analyzing phylogenetic relationship of MBD gene families from Arabidopsis, rice, wheat, and maize, all 14 MBD proteins in maize were categorized into four subclasses. Furthermore, chromosome location and schematic structure revealed an unevenly distribution on chromosomes and structure features of MBD genes in maize, respectively. Eventually, EST expression data mining, microarray data clustering analysis, and semi-quantitative and quantitative expression profile analyses detected in seedling leaves and stems by heat, drought, and salt-stress treatments have demonstrated that these genes had temporal and spatial expression pattern and exhibited different expression levels under heat, drought, and salt-stress conditions, suggesting that functional diversification of the MBD gene family in maize. In addition, through electrophoretic mobility shift assay (EMSA) a representative MBD protein, ZmMBD11, exhibited in vitro DNA-binding activity, indicating that that the MBD proteins in maize might play a role in reading cytosine methylation. Taken together, these results would provide an important theoretical basis for future functional verification of ZmMBD genes and also facilitate future experimental research to further unravel the mechanisms of epigenetic regulation in plants. |
| 资助项目 | National Natural Science Foundation of China[31571673] ; Anhui Provincial University Excellent Youth Talent Support Program[06146007] ; National Engineering Laboratory of Crop Stress Resistance Breeding[KNZJ1023] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000515945600002 |
| 出版者 | SPRINGER |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14930]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Qian, Yexiong |
| 作者单位 | 1.Anhui Normal Univ, Anhui Prov Key Lab Conservat & Exploitat Biol Res, Wuhu 241000, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qian, Yexiong,Ren, Qiaoyu,Jiang, Lingyu,et al. Genome-wide analysis of maize MBD gene family and expression profiling under abiotic stress treatment at the seedling stage[J]. PLANT BIOTECHNOLOGY REPORTS,2020:16. |
| APA | Qian, Yexiong,Ren, Qiaoyu,Jiang, Lingyu,Zhang, Jing,Chen, Changle,&Chen, Liang.(2020).Genome-wide analysis of maize MBD gene family and expression profiling under abiotic stress treatment at the seedling stage.PLANT BIOTECHNOLOGY REPORTS,16. |
| MLA | Qian, Yexiong,et al."Genome-wide analysis of maize MBD gene family and expression profiling under abiotic stress treatment at the seedling stage".PLANT BIOTECHNOLOGY REPORTS (2020):16. |
入库方式： OAI收割
来源：武汉植物园
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