Headspace gas chromatographic method for antimicrobial screening: Minimum inhibitory concentration determination
文献类型：期刊论文
| 作者 | Li, Menghui5,6; Zhang, Chunyun3,5,7; Chen, Guilin3,5,7; Nahar, Lutfun2,4; Sarker, Satyajit D.1; Guo, Mingquan3,5,7 |
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2020-03-20 |
| 卷号 | 181页码:6 |
| 关键词 | Antimicrobial activity Headspace analysis Metabolic carbon dioxide Natural products Minimum inhibitory concentration |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2020.113122 |
| 英文摘要 | The headspace gas chromatographic method has been widely used to detect volatile metabolites to reflect the growth state of microorganisms, however, it has never been used for the determination of the minimum inhibitory concentration in antibacterial drugs. This paper reports a new method for evaluating the antimicrobial activity of drugs by measuring the amount of CO2 produced by bacterial metabolism after treatment with drugs. According to the amount of CO2 produced by bacterial metabolism, a proper amount of bacterial liquid is selected and added to a drug-containing culture medium as compared with bacteria without drugs in parallel. The amount of CO2 produced by bacteria is measured by using a headspace gas chromatograph coupled with a thermal conductivity detector to measure the bacteriostasis rate and the minimum bacteriostasis concentration of the tested drug, so as to evaluate its antibacterial activity. The accuracy of this method was verified by comparison with the standard method (the OD method), which indicated that the precision was less than 3 % (expressed by relative standard deviation), the inhibition rate (R-2 = 0.968) was consistent with the reference method above. This method is simple in operation and can avoid the error caused by the properties of the sample such as volatility, solubility and color in the determination of the minimum inhibitory concentration. It is suitable for the determination of antibacterial activity of drugs, especially natural drugs. (C) 2020 Elsevier B.V. All rights reserved. |
| 资助项目 | Major Project for Special Technology Innovation of Hubei Province[2017AHB054] ; European Regional Development Fund Project ENOCH[CZ.02.1.01/0.0/0.0/16_019/0000868] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000516107400002 |
| 出版者 | ELSEVIER |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/14986]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Mingquan |
| 作者单位 | 1.Liverpool John Moores Univ, Ctr Nat Prod Discovery, Sch Pharm & Biomol Sci, James Parsons Bldg,Byrom St, Liverpool L3 3AF, Merseyside, England 2.Palacky Univ, Slechtitelu 27, Olomouc 78371, Czech Republic 3.Chinese Acad Sci, Innovat Acad Drug Discovery & Dev, Shanghai 201203, Peoples R China 4.Inst Expt Bot ASCR, Lab Growth Regulators, Slechtitelu 27, Olomouc 78371, Czech Republic 5.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Menghui,Zhang, Chunyun,Chen, Guilin,et al. Headspace gas chromatographic method for antimicrobial screening: Minimum inhibitory concentration determination[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2020,181:6. |
| APA | Li, Menghui,Zhang, Chunyun,Chen, Guilin,Nahar, Lutfun,Sarker, Satyajit D.,&Guo, Mingquan.(2020).Headspace gas chromatographic method for antimicrobial screening: Minimum inhibitory concentration determination.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,181,6. |
| MLA | Li, Menghui,et al."Headspace gas chromatographic method for antimicrobial screening: Minimum inhibitory concentration determination".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 181(2020):6. |
入库方式： OAI收割
来源：武汉植物园
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