The NAD kinase OsNADK1 affects the intracellular redox balance and enhances the tolerance of rice to drought
文献类型：期刊论文
| 作者 | Wang, Xiang1,3; Li, Bin-Bin3; Ma, Tian-Tian3; Sun, Liang-Yu3; Tai, Li3; Hu, Chun-Hong2,3; Liu, Wen-Ting3; Li, Wen-Qiang3; Chen, Kun-Ming3 |
| 刊名 | BMC PLANT BIOLOGY
 |
| 出版日期 | 2020-01-07 |
| 卷号 | 20期号:1页码:19 |
| 关键词 | NAD kinase OsNADK1 Intracellular redox balance OsDREB1B Proline Drought tolerance Rice (Oryza sativa) |
| ISSN号 | 1471-2229 |
| DOI | 10.1186/s12870-019-2234-8 |
| 英文摘要 | Background NAD kinases (NADKs) are the only known enzymes that directly phosphorylate NAD(H) to generate NADP(H) in different subcellular compartments. They participate in multiple life activities, such as modulating the NADP/NAD ratio, maintaining the intracellular redox balance and responding to environmental stresses. However, the functions of individual NADK in plants are still under investigation. Here, a rice NADK, namely, OsNADK1, was identified, and its functions in plant growth regulation and stress tolerance were analysed by employing a series of transgenic plant lines. Results OsNADK1 is a cytosol-localized NADK in rice. It was expressed in all rice tissues examined, and its transcriptional expression could be stimulated by a number of environmental stress treatments. Compared with wild-type (WT) rice, the mutant plant osnadk1 in which OsNADK1 was knocked out was a dwarf at the heading stage and had decreased NADP(H)/NAD(H), ascorbic acid (ASA)/dehydroascorbate (DHA) and reduced glutathione (GSH)/oxidized glutathione (GSSG) ratios, which led to increased oxidation states in the rice cells and sensitivity to drought. Moreover, certain stress-related genes showed differential expression patterns in osnadk1 under both normal growth and drought-stress conditions compared with WT. Among these genes, OsDREB1B and several WRKY family transcription factors, e.g., OsWRKY21 and OsWRKY42, showed correlated co-expression patterns with OsNADK1 in osnadk1 and the plants overexpressing or underexpressing OsNADK1, implying roles for these transcription factors in OsNADK1-mediated processes. In addition, overexpression of OsNADK1 enhanced the drought tolerance of rice plants, whereas loss of function of the gene reduced the tolerance. Furthermore, the proline content was dramatically increased in the leaves of the OsNADK1-overexpressing lines under drought conditions. Conclusions Altogether, the results suggest that an OsNADK1-mediated intracellular redox balance is involved in the tolerance of rice plants to drought. |
| 资助项目 | National Natural Science Foundation of China[31770204] ; National Natural Science Foundation of China[31270299] ; Programme for New Century Excellent Talents in Universities of China[NCET-11-0440] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000521245200001 |
| 出版者 | BMC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15082]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chen, Kun-Ming |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan 430074, Peoples R China 2.Zhoukou Normal Univ, Coll Life Sci & Agr, Zhoukou 466001, Henan, Peoples R China 3.Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Area, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiang,Li, Bin-Bin,Ma, Tian-Tian,et al. The NAD kinase OsNADK1 affects the intracellular redox balance and enhances the tolerance of rice to drought[J]. BMC PLANT BIOLOGY,2020,20(1):19. |
| APA | Wang, Xiang.,Li, Bin-Bin.,Ma, Tian-Tian.,Sun, Liang-Yu.,Tai, Li.,...&Chen, Kun-Ming.(2020).The NAD kinase OsNADK1 affects the intracellular redox balance and enhances the tolerance of rice to drought.BMC PLANT BIOLOGY,20(1),19. |
| MLA | Wang, Xiang,et al."The NAD kinase OsNADK1 affects the intracellular redox balance and enhances the tolerance of rice to drought".BMC PLANT BIOLOGY 20.1(2020):19. |
入库方式： OAI收割
来源：武汉植物园
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