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CER16 Inhibits Post-Transcriptional Gene Silencing of CER3 to Regulate Alkane Biosynthesis(1)([OPEN])

文献类型：期刊论文

作者Yang, Xianpeng2,4; Feng, Tao4; Li, Shipeng4,5; Zhao, Huayan1; Zhao, Shuangshuang6; Ma, Changle6; Jenks, Matthew A.3; Lu, Shiyou1
刊名PLANT PHYSIOLOGY
出版日期2020-03-01
卷号182期号:3页码:1211-1221
ISSN号0032-0889
DOI10.1104/pp.19.01002
英文摘要The aerial surfaces of land plants have a protective layer of cuticular wax. Alkanes are common components of these waxes, and their abundance is affected by a range of stresses. The CER16 protein has been implicated in alkane biosynthesis in the cuticular wax of Arabidopsis (Arabidopsis thaliana). Here, we identified two new mutant alleles of CER16 in Arabidopsis resulting in production of less wax with dramatically fewer alkanes than the wild type. Map-based cloning with genetic analysis revealed that the cer16 phenotype was caused by complete loss of AT5G44150, encoding a protein with no known domains or motifs. Comparative transcriptomic analysis revealed that transcripts of CER3, previously shown to play a principal role in alkane production, were markedly reduced in the cer16 mutants. To define the relationship between CER3 and CER16, we transformed the full CER3 gene into a cer16 mutant. Transgenic CER3 expression was silenced, and levels of small interfering RNAs targeting CER3 were significantly increased. Mutating two major components of the RNA-silencing machinery in a cer16 genetic background restored CER3 transcript levels to wild-type levels, with the stems restored to wild-type glaucousness. We suggest that CER16 deficiency induces post-transcriptional gene silencing of both endogenous and exogenous expression of CER3. Alkane biosynthesis in cuticular wax of Arabidopsis associated with stress responses is regulated through an RNA quality control mechanism.
资助项目National Natural Science Foundation of China[31570186]
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000519970600005
出版者AMER SOC PLANT BIOLOGISTS
源URL[http://202.127.146.157/handle/2RYDP1HH/15094]  
专题中国科学院武汉植物园
通讯作者Lu, Shiyou
作者单位1.Hubei Univ, State Key Lab Biocatalysis & Enzyme Engn, Sch Life Sci, Wuhan 434200, Peoples R China
2.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China
3.Univ Arizona, Sch Plant Sci, Coll Agr & Life Sci, Tucson, AZ 85721 USA
4.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Xianpeng,Feng, Tao,Li, Shipeng,et al. CER16 Inhibits Post-Transcriptional Gene Silencing of CER3 to Regulate Alkane Biosynthesis(1)([OPEN])[J]. PLANT PHYSIOLOGY,2020,182(3):1211-1221.
APA Yang, Xianpeng.,Feng, Tao.,Li, Shipeng.,Zhao, Huayan.,Zhao, Shuangshuang.,...&Lu, Shiyou.(2020).CER16 Inhibits Post-Transcriptional Gene Silencing of CER3 to Regulate Alkane Biosynthesis(1)([OPEN]).PLANT PHYSIOLOGY,182(3),1211-1221.
MLA Yang, Xianpeng,et al."CER16 Inhibits Post-Transcriptional Gene Silencing of CER3 to Regulate Alkane Biosynthesis(1)([OPEN])".PLANT PHYSIOLOGY 182.3(2020):1211-1221.

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来源：武汉植物园

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