Genome-Wide Identification and Expression Analysis of Polygalacturonase Gene Family in Kiwifruit (Actinidia chinensis) during Fruit Softening
文献类型：期刊论文
| 作者 | Huang, Wenjun1,2,3; Chen, Meiyan1,2,3; Zhao, Tingting1,2,3; Han, Fei1,2,3; Zhang, Qi1,2,3; Liu, Xiaoli1,2,3; Jiang, Changying2,3; Zhong, Caihong1,2,3 |
| 刊名 | PLANTS-BASEL
 |
| 出版日期 | 2020-03-01 |
| 卷号 | 9期号:3页码:16 |
| 关键词 | kiwifruit Actinidia polygalacturonase pectin cell wall softening |
| DOI | 10.3390/plants9030327 |
| 英文摘要 | Polygalacturonase (PG) is an essential hydrolytic enzyme responsible for pectin degradation and thus plays an important role in fruit softening and other cell separation processes. PG protein is encoded by a multigene family, however, the members of PG gene family in kiwifruit (Actinidia chinensis) have not been extensively identified. In this study, a total of 51 AcPG genes in kiwifruit genome were identified. They are phylogenetically clustered into seven clades, and of them AcPG4 and AcPG18 with other known PG genes involved in fruit softening from peach, pear, papaya and melon form a small cluster together. The members of kiwifruit PG gene family consist of three to nine exons and two to eight introns, and their exon/intron structures are generally conserved in all clades except the clade D and E. During fruit softening of kiwifruit 'Donghong' under ambient temperature, cell wall modifying enzymes, including PG, PL (pectate and pectin lyases), and PE (pectinesterase, also known as pectin methylesterase, PME) showed a different activity profile, and of them, PG and PE activities largely correlated with the change of pectin content and firmness. Moreover, only 11 AcPG genes were highly or moderately expressed in softening fruit, and of which three AcPG genes (AcPG4, AcPG18, and AcPG8, especially the former two) has been found to strongly correlate with the profile of PG activity and pectin content, as well as fruit firmness, suggesting that they maybe play an important role in fruit softening. Thus, our findings not only benefit the functional characterization of kiwifruit PG genes, but also provide a subset of potential PG candidate genes for further genetic manipulation. |
| 资助项目 | Strategic Priority Research Program of Chinese Academy of Sciences[XDA24030404] ; Technological Innovation Project of Hubei Province (Key Program)[2016ABA109] ; National Key Research and Development Program of China[2018YFD1000105] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000525315000041 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15118]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhong, Caihong |
| 作者单位 | 1.Chinese Acad Sci, Engn Lab Kiwifruit Ind Technol, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Wenjun,Chen, Meiyan,Zhao, Tingting,et al. Genome-Wide Identification and Expression Analysis of Polygalacturonase Gene Family in Kiwifruit (Actinidia chinensis) during Fruit Softening[J]. PLANTS-BASEL,2020,9(3):16. |
| APA | Huang, Wenjun.,Chen, Meiyan.,Zhao, Tingting.,Han, Fei.,Zhang, Qi.,...&Zhong, Caihong.(2020).Genome-Wide Identification and Expression Analysis of Polygalacturonase Gene Family in Kiwifruit (Actinidia chinensis) during Fruit Softening.PLANTS-BASEL,9(3),16. |
| MLA | Huang, Wenjun,et al."Genome-Wide Identification and Expression Analysis of Polygalacturonase Gene Family in Kiwifruit (Actinidia chinensis) during Fruit Softening".PLANTS-BASEL 9.3(2020):16. |
入库方式： OAI收割
来源：武汉植物园
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