The ethylene responsive factor CdERF1 from bermudagrass (Cynodon dactylon) positively regulates cold tolerance
文献类型：期刊论文
| 作者 | Hu, Zhengrong1,4; Huang, Xuebing1; Amombo, Erick1; Liu, Ao1; Fan, Jibiao3; Bi, Aoyue1; Ji, Kang1; Xin, Haiping1; Chen, Liang1; Fu, Jinmin1,2 |
| 刊名 | PLANT SCIENCE
 |
| 出版日期 | 2020-05-01 |
| 卷号 | 294页码:13 |
| ISSN号 | 0168-9452 |
| 关键词 | Bermudagrass CdERF1 Cold stress tolerance Lipid transfer protein Peroxidase Transcriptional regulation |
| DOI | 10.1016/j.plantsci.2020.110432 |
| 英文摘要 | Cold stress is one of the major environmental factors that limit growth and utilization of bermudagrass [Cynodon dactylon (L.) Pers], a prominent warm-season turfgrass. However, the molecular mechanism of cold response in bermudagrass remains largely unknown. In this study, we characterized a cold-responsive ERF (ethylene responsive factor) transcription factor, CdERF1, from bermudagrass. CdERF1 expression was induced by cold, drought and salinity stresses. The CdERF1 protein was nucleus-localized and encompassed transcriptional activation activity. Transgenic Arabidopsis plants overexpressing CdERF1 showed enhanced cold tolerance, whereas CdERF1-underexpressing bermudagrass plants via virus induced gene silencing (VIGS) method exhibited reduced cold resistance compared with control, respectively. Under cold stress, electrolyte leakage (EL), malondialdehyde (MDA), H2O2 and O2- contents were reduced, while the activities of SOD and POD were elevated in transgenic Arabidopsis. By contrast, these above physiological indicators in CdERF1-underexpressing bermudagrass exhibited the opposite trend. To further explore the possible molecular mechanism of bermudagrass cold stress response, the RNA-Seq analyses were performed. The result indicated that overexpression of CdERF1 activated a subset of stress-related genes in transgenic Arabidopsis, such as CBF2, pEARLI1 (lipid transfer protein), PER71 (peroxidase) and LTP (lipid transfer protein). Interestingly, under-expression of CdERF1 suppressed the transcription of many genes in CdERF1-underexpressing bermudagrass, also including pEARLI1 (lipid transfer protein) and PER70 (peroxidase). All these results revealed that CdERF1 positively regulates plant cold response probably by activating stress-related genes, PODs, CBF2 and LTPs. This study also suggests that CdERF1 may be an ideal candidate in the effort to improve cold tolerance of bermudagrass in the further molecular breeding. |
| 资助项目 | National Natural Science Foundation of China (NSFC)[31272194] ; National Natural Science Foundation of China (NSFC)[31672482] ; National Natural Science Foundation of China (NSFC)[31401915] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| 出版者 | ELSEVIER IRELAND LTD |
| WOS记录号 | WOS:000528164300003 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15181]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chen, Liang; Fu, Jinmin |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Ludong Univ, Shandong Coastal Salin Tolerant Grass Engn & Tech, Yantai 264025, Peoples R China 3.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China 4.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Zhengrong,Huang, Xuebing,Amombo, Erick,et al. The ethylene responsive factor CdERF1 from bermudagrass (Cynodon dactylon) positively regulates cold tolerance[J]. PLANT SCIENCE,2020,294:13. |
| APA | Hu, Zhengrong.,Huang, Xuebing.,Amombo, Erick.,Liu, Ao.,Fan, Jibiao.,...&Fu, Jinmin.(2020).The ethylene responsive factor CdERF1 from bermudagrass (Cynodon dactylon) positively regulates cold tolerance.PLANT SCIENCE,294,13. |
| MLA | Hu, Zhengrong,et al."The ethylene responsive factor CdERF1 from bermudagrass (Cynodon dactylon) positively regulates cold tolerance".PLANT SCIENCE 294(2020):13. |
入库方式： OAI收割
来源：武汉植物园
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