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Functional Analysis Reveals the Regulatory Role of PpTST1 Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit

文献类型：期刊论文

作者Peng, Qian2,4; Wang, Lu2,3; Ogutu, Collins1,2,4; Liu, Jingjing2,4; Liu, Li2,4; Mollah, Md Dulal Ali2,4; Han, Yuepeng1,2,3
刊名INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
出版日期2020-02-01
卷号21期号:3页码:11
关键词Prunus persica tonoplast sugar transporter dCAPS QTL fruit sweetness
DOI10.3390/ijms21031112
英文摘要Sugar content is related to fruit sweetness, and the complex mechanisms underlying fruit sugar accumulation still remain elusive. Here, we report a peach PpTST1 gene encoding tonoplast sugar transporter that is located in the quantitative trait loci (QTL) interval on Chr5 controlling fruit sucrose content. One derived Cleaved Amplified Polymorphic Sequence (dCAPS) marker was developed based on a nonsynonymous G/T variant in the third exon of PpTST1. Genotyping of peach cultivars with the dCAPS marker revealed a significant difference in fruit sugar content among genotypes. PpTST1 is located in the tonoplast, and substitution of glutamine by histidine caused by the G/T variation has no impact on subcellular location. The expression profile of PpTST1 exhibited a consistency with the sugar accumulation pattern, and its transient silencing significantly inhibited sugar accumulation in peach fruits. All of these results demonstrated the role of PpTST1 in regulating sugar accumulation in peach fruit. In addition, cis-elements for binding of MYB and WRKY transcript factors were found in the promoter sequence of PpTST1, suggesting a gene regulatory network of fruit sugar accumulation. Our results are not only helpful for understanding the mechanisms underlying fruit sugar accumulation, but will also be useful for the genetic improvement of fruit sweetness in peach breeding programs.
资助项目National Key Research and Development Program[2018YFD1000200] ; National Natural Science Foundation of China[31872087] ; China Agriculture Research System[CARS-30]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000522551607039
出版者MDPI
源URL[http://202.127.146.157/handle/2RYDP1HH/15183]  
专题中国科学院武汉植物园
通讯作者Han, Yuepeng
作者单位1.Chinese Acad Sci, Sino African Joint Res Ctr, Wuhan 430074, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Peoples R China
3.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Wuhan 430074, Peoples R China
4.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Peng, Qian,Wang, Lu,Ogutu, Collins,et al. Functional Analysis Reveals the Regulatory Role of PpTST1 Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(3):11.
APA Peng, Qian.,Wang, Lu.,Ogutu, Collins.,Liu, Jingjing.,Liu, Li.,...&Han, Yuepeng.(2020).Functional Analysis Reveals the Regulatory Role of PpTST1 Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(3),11.
MLA Peng, Qian,et al."Functional Analysis Reveals the Regulatory Role of PpTST1 Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.3(2020):11.

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来源：武汉植物园

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