A phase conversion headspace technique for the determination of anti-anaerobic activity of drug candidate based on the metabolic acidity change in culture medium
文献类型：期刊论文
| 作者 | Zhang, Chun-Yun1,2,3,4; Li, Meng-Hui2,3; Guo, Ming-Quan1,2,3,4 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2020-06-21 |
| 卷号 | 1621页码:6 |
| 关键词 | Drug screening Anti-anaerobic activity Metabolic acidity Headspace-gas chromatography |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2020.461024 |
| 英文摘要 | Screening for anti-anaerobic drug candidates is still challenging although the anaerobic bacteria are important sources for human infections, because the method for anti-anaerobic activity testing is not readily available with low-cost and -expertise. We report a novel method for the determination of the anti-anaerobic activity of drug candidates by automated headspace-gas chromatography (HS-GC). Anaerobic bacteria were inoculated in an anaerobic atmosphere or rapidly using sterile syringe in an air-tight manner, and incubated with and without drugs for 48 h. The metabolic acidities of the cultured media were used as an indicator of cell activities and measured as end-products in place by HS-GC after being completely converted to CO2 with sodium bicarbonate. The present method is precise (relative standard deviation is below 5%) and validated by excellent agreements with a reference method on the determinations of the inhibition rates (root-mean-square error = 10%, n = 48) and half maximal inhibitory concentrations (R-2 = 0.996, n = 8) of both pure drug compounds and plant extracts. Advantageously, the present method is sensitive in response to cell activity, safe with regard to cross contamination, and suitable for routine screening of diversified drug candidates for anti-anaerobic activity. (C) 2020 Elsevier B.V. All rights reserved. |
| 资助项目 | Natural Science Foundation of China[31601800] ; Major Project for Special Technology Innovation of Hubei Province-Sino-foreign CooperationProject[2017AHB054] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000534277500004 |
| 出版者 | ELSEVIER |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15278]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Ming-Quan |
| 作者单位 | 1.Chinese Acad Sci, Sino African Joint Res Ctr, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan 430074, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Innovat Acad Drug Discovery & Dev, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Chun-Yun,Li, Meng-Hui,Guo, Ming-Quan. A phase conversion headspace technique for the determination of anti-anaerobic activity of drug candidate based on the metabolic acidity change in culture medium[J]. JOURNAL OF CHROMATOGRAPHY A,2020,1621:6. |
| APA | Zhang, Chun-Yun,Li, Meng-Hui,&Guo, Ming-Quan.(2020).A phase conversion headspace technique for the determination of anti-anaerobic activity of drug candidate based on the metabolic acidity change in culture medium.JOURNAL OF CHROMATOGRAPHY A,1621,6. |
| MLA | Zhang, Chun-Yun,et al."A phase conversion headspace technique for the determination of anti-anaerobic activity of drug candidate based on the metabolic acidity change in culture medium".JOURNAL OF CHROMATOGRAPHY A 1621(2020):6. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。