Development and characterization of EST-SSR markers for the endangered tree Magnolia patungensis (Magnoliaceae)
文献类型：期刊论文
| 作者 | Wagutu, Godfrey K.1,2,3; Fan, Xiang-Rong2,4,5; Njeri, Henry K.1,2,3; Wen, Xiang-Ying6; Liu, Yan-Ling2; Chen, Yuan-Yuan2 |
| 刊名 | ANNALES BOTANICI FENNICI
 |
| 出版日期 | 2020-05-01 |
| 卷号 | 57期号:1-3页码:97-107 |
| ISSN号 | 0003-3847 |
| DOI | 10.5735/085.057.0114 |
| 英文摘要 | Magnolia patungensis (Magnoliaceae) is an endangered tree species endemic to China. To facilitate analysis of its genetic variability, we developed novel polymorphic expressed sequence tag-simple sequence repeat (EST-SSR) markers using transcriptome data. In total, 37 774 SSRs amounting to 91.52 Mb were identified from 131 132 unigenes, with the frequency of 28.81% and the average density of 2.42 kb. Mononucleotide (50.81%) and dinucleotide (34.12%) were the most abundant repeat types. We randomly selected 102 putative loci to test the amplification efficiency and the degree of polymorphism using three populations from which 15 individuals per population were sampled. The successful amplification rate was 36.27% and the polymorphism rate 12.75%. The 13 polymorphic SSR marker primers selected were used to characterize the genetic diversity of M. patungensis. A total of 40 alleles were identified for the species, ranging from 2 to 5 per locus. At the population level, the mean number of alleles per locus ranged from 1.9 to 2.5. and the mean observed and expected heterozygosity values from 0.308 to 0.446 and 0.304 to 0.424. respectively. Significant genetic differentiation (F-ST = 0.286) and low gene flow (N-m = 0.627) among populations were discovered. Transferability of the markers was tested in five congeneric species. Nine loci were successfully amplified in M. dandyi, eight in M. insignis, M. fordiana and M. hookeri, and seven in M. conifera. These new EST-SSR markers will be an essential tool for the conservation of this endangered species as well as for investigating the evolutionary history forts. patungensis and its congenerics. |
| 资助项目 | Botanic Gardens Conservation International (BGCI)[30420] ; China's Ministry of Science & Technology under Grant of the National Key R&D Program of China[2016YFA0601001] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000535267000014 |
| 出版者 | FINNISH ZOOLOGICAL BOTANICAL PUBLISHING BOARD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15289]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chen, Yuan-Yuan |
| 作者单位 | 1.Chinese Acad Sci, Sino Africa Joint Res Ctr, CN-430074 Wuhan, Peoples R China 2.Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, CN-430074 Wuhan, Peoples R China 3.Univ Chinese Acad Sci, CN-100049 Beijing, Peoples R China 4.Tibet Univ, Coll Sci, CN-850000 Lhasa, Tibet Autonomou, Peoples R China 5.Tibet Univ, Res Ctr Ecol & Environm Qinghai Tibetan Plateau, CN-850000 Lhasa, Tibet Autonomou, Peoples R China 6.Chinese Acad Sci, South China Bot Garden, 723 Xingke Rd, CN-510650 Guangzhou, Tianhe District, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wagutu, Godfrey K.,Fan, Xiang-Rong,Njeri, Henry K.,et al. Development and characterization of EST-SSR markers for the endangered tree Magnolia patungensis (Magnoliaceae)[J]. ANNALES BOTANICI FENNICI,2020,57(1-3):97-107. |
| APA | Wagutu, Godfrey K.,Fan, Xiang-Rong,Njeri, Henry K.,Wen, Xiang-Ying,Liu, Yan-Ling,&Chen, Yuan-Yuan.(2020).Development and characterization of EST-SSR markers for the endangered tree Magnolia patungensis (Magnoliaceae).ANNALES BOTANICI FENNICI,57(1-3),97-107. |
| MLA | Wagutu, Godfrey K.,et al."Development and characterization of EST-SSR markers for the endangered tree Magnolia patungensis (Magnoliaceae)".ANNALES BOTANICI FENNICI 57.1-3(2020):97-107. |
入库方式： OAI收割
来源：武汉植物园
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