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Influence of metals and metalloids on the composition and fluorescence quenching of the extracellular polymeric substances produced by the polymorphic fungusAureobasidium pullulans

文献类型：期刊论文

作者Song, Wenjuan2,3; Yang, Yuyi3,4; Liang, Xinjin3,5; Liu, Feixue3; Gadd, Geoffrey Michael1,3
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2020-06-23
页码10
关键词Mercury Selenium Aureobasidium pullulans Excitation-emission matrix (EEM) fluorescence spectra Extracellular polymeric substances (EPS)
ISSN号0175-7598
DOI10.1007/s00253-020-10732-7
英文摘要Aureobasidium pullulansis a ubiquitous and widely distributed fungus in the environment, and exhibits substantial tolerance against toxic metals. However, the interactions between metals and metalloids with the copious extracellular polymeric substances (EPS) produced byA. pullulansand possible relationships to tolerance are not well understood. In this study, it was found that mercury (Hg) and selenium (Se), as selenite, not only significantly inhibited growth ofA. pullulansbut also affected the composition of produced EPS. Lead (Pb) showed little influence on EPS yield or composition. The interactions of EPS fromA. pullulanswith the tested metals and metalloids depended on the specific element and their concentration. Fluorescence intensity measurements of the EPS showed that the presence of metal(loid)s stimulated the production of extracellular tryptophan-like and aromatic protein-like substances. Examination of fluorescence quenching and calculation of binding constants revealed that the fluorescence quenching process for Hg; arsenic (As), as arsenite; and Pb to EPS were mainly governed by static quenching which resulted in the formation of a stable non-fluorescent complexes between the EPS and metal(loid)s. Se showed no significant interaction with the EPS according to fluorescence quenching. These results provide further understanding of the interactions between metals and metalloids and EPS produced by fungi and their contribution to metal(loid) tolerance.
资助项目National Natural Science Foundation of China[41673127] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2017478] ; Natural Environment Research Council[NE/M010910/1] ; Natural Environment Research Council[NE/M011275/1 (COG3)]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000542502600002
出版者SPRINGER
源URL[http://202.127.146.157/handle/2RYDP1HH/15397]  
专题中国科学院武汉植物园
通讯作者Gadd, Geoffrey Michael
作者单位1.China Univ Petr, Coll Chem Engn & Environm, Beijing Key Lab Oil & Gas Pollut Control, State Key Lab Heavy Oil Proc, Beijing 102249, Peoples R China
2.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China
3.Univ Dundee, Sch Life Sci, Geomicrobiol Grp, Dundee DD1 5EH, Scotland
4.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Aquat Bot & Watershed Ecol, Wuhan 430074, Peoples R China
5.Univ South Wales, Sustainable Environm Res Ctr, Pontypridd CF37 4BD, M Glam, Wales
推荐引用方式
GB/T 7714		 Song, Wenjuan,Yang, Yuyi,Liang, Xinjin,et al. Influence of metals and metalloids on the composition and fluorescence quenching of the extracellular polymeric substances produced by the polymorphic fungusAureobasidium pullulans[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020:10.
APA Song, Wenjuan,Yang, Yuyi,Liang, Xinjin,Liu, Feixue,&Gadd, Geoffrey Michael.(2020).Influence of metals and metalloids on the composition and fluorescence quenching of the extracellular polymeric substances produced by the polymorphic fungusAureobasidium pullulans.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,10.
MLA Song, Wenjuan,et al."Influence of metals and metalloids on the composition and fluorescence quenching of the extracellular polymeric substances produced by the polymorphic fungusAureobasidium pullulans".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020):10.

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来源：武汉植物园

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