In-Depth Investigation of Low-Abundance Proteins in Matured and Filling Stages Seeds ofGlycine maxEmploying a Combination of Protamine Sulfate Precipitation and TMT-Based Quantitative Proteomic Analysis
文献类型：期刊论文
| 作者 | Min, Cheol Woo2; Park, Joonho1; Bae, Jin Woo3; Agrawal, Ganesh Kumar4,5; Rakwal, Randeep4,5,6; Kim, Youngsoo1; Yang, Pingfang7; Kim, Sun Tae2; Gupta, Ravi2,8 |
| 刊名 | CELLS
 |
| 出版日期 | 2020-06-01 |
| 卷号 | 9期号:6页码:20 |
| 关键词 | soybean basic pH reverse phase filter-aided sample preparation low-abundance proteins maxquant protamine sulfate precipitation perseus tandem mass tags |
| DOI | 10.3390/cells9061517 |
| 英文摘要 | Despite the significant technical advancements in mass spectrometry-based proteomics and bioinformatics resources, dynamic resolution of soybean seed proteome is still limited because of the high abundance of seed storage proteins (SSPs). These SSPs occupy a large proportion of the total seed protein and hinder the identification of low-abundance proteins. Here, we report a TMT-based quantitative proteome analysis of matured and filling stages seeds of high-protein (Saedanbaek) and low-protein (Daewon) soybean cultivars by application of a two-way pre-fractionation both at the levels of proteins (by PS) and peptides (by basic pH reverse phase chromatography). Interestingly, this approach led to the identification of more than 5900 proteins which is the highest number of proteins reported to date from soybean seeds. Comparative protein profiles of Saedanbaek and Daewon led to the identification of 2200 and 924 differential proteins in mature and filling stages seeds, respectively. Functional annotation of the differential proteins revealed enrichment of proteins related to major metabolism including amino acid, major carbohydrate, and lipid metabolism. In parallel, analysis of free amino acids and fatty acids in the filling stages showed higher contents of all the amino acids in the Saedanbaek while the fatty acids contents were found to be higher in the Daewon. Taken together, these results provide new insights into proteome changes during filling stages in soybean seeds. Moreover, results reported here also provide a framework for systemic and large-scale dissection of seed proteome for the seeds rich in SSPs by two-way pre-fractionation combined with TMT-based quantitative proteome analysis. |
| 资助项目 | National Research Foundation of Korea (NRF) through the leading research supporting program[2019R1A2C2085868] ; Rural Development Administration (RDA) through Systems & Synthetic Agrobiotech Center (SSAC)[PJ013186032020] ; National Research Foundation of Korea (NRF) through the next generation supporting program[2019R1A6A3A13095655] ; Ramalingaswami re-entry fellowship from the Department of Biotechnology, Ministry of Science and Technology, Government of India[BT/HRD/35/02/2006] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000550781100001 |
| 出版者 | MDPI |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15470]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Kim, Sun Tae; Gupta, Ravi |
| 作者单位 | 1.Seoul Natl Univ, Coll Engn, Interdisciplinary Program Bioengn, Seoul 03080, South Korea 2.Pusan Natl Univ, Dept Plant Biosci, Miryang 50463, South Korea 3.Rural Dev Adm, Natl Inst Crop Sci, Wonju 55365, South Korea 4.Res Lab Biotechnol & Biochem RLABB, GPO 13265, Kathmandu 44600, Nepal 5.GRADE Global Res Arch Developing Educ Acad Privat, Adarsh Nagar 13, Birgunj 44300, Nepal 6.Univ Tsukuba, Fac Hlth & Sport Sci, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058574, Japan 7.Chinese Acad Sci, China Ncsacgbw Fpgnay, Wuhan Bot Garden, Wuhan 430074, Peoples R China 8.Jamia Hamdard, Dept Bot, Sch Chem & Life Sci, New Delhi 110062, India |
| 推荐引用方式 GB/T 7714 | Min, Cheol Woo,Park, Joonho,Bae, Jin Woo,et al. In-Depth Investigation of Low-Abundance Proteins in Matured and Filling Stages Seeds ofGlycine maxEmploying a Combination of Protamine Sulfate Precipitation and TMT-Based Quantitative Proteomic Analysis[J]. CELLS,2020,9(6):20. |
| APA | Min, Cheol Woo.,Park, Joonho.,Bae, Jin Woo.,Agrawal, Ganesh Kumar.,Rakwal, Randeep.,...&Gupta, Ravi.(2020).In-Depth Investigation of Low-Abundance Proteins in Matured and Filling Stages Seeds ofGlycine maxEmploying a Combination of Protamine Sulfate Precipitation and TMT-Based Quantitative Proteomic Analysis.CELLS,9(6),20. |
| MLA | Min, Cheol Woo,et al."In-Depth Investigation of Low-Abundance Proteins in Matured and Filling Stages Seeds ofGlycine maxEmploying a Combination of Protamine Sulfate Precipitation and TMT-Based Quantitative Proteomic Analysis".CELLS 9.6(2020):20. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。