GDSL lipase occluded stomatal pore 1 is required for wax biosynthesis and stomatal cuticular ledge formation
文献类型：期刊论文
| 作者 | Tang, Jing3; Yang, Xianpeng2; Xiao, Chuanlei3; Li, Jiaying3; Chen, Yongqiang3; Li, Ruiying3; Li, Shipeng2; Lu, Shiyou1; Hu, Honghong3 |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2020-07-24 |
| 页码 | 17 |
| 关键词 | Arabidopsis drought tolerance epidermal permeability OSP1 stomatal outer cuticular ledge thioesterase activity water loss wax biosynthesis |
| ISSN号 | 0028-646X |
| DOI | 10.1111/nph.16741 |
| 英文摘要 | The plant leaf surface is coated with a waterproof cuticle layer. Cuticle facing the stomatal pore surface needs to be sculpted to form outer cuticular ledge (OCL) after stomatal maturation for efficient gas exchange. Here, we characterized the roles of Arabidopsis GDSL lipase, Occlusion of Stomatal Pore 1 (OSP1), in wax biosynthesis and stomatal OCL formation. OSP1mutation results in significant reduction in leaf wax synthesis and occlusion of stomata, leading to increased epidermal permeability, decreased transpiration rate, and enhanced drought tolerance. We demonstrated that OSP1 activity is critical for its role in wax biosynthesis and stomatal function.In vitroenzymatic assays demonstrated that OSP1 possesses thioesterase activity, particularly on C22:0 and C26:0 acyl-CoAs. Genetic interaction analyses withCER1(ECERIFERUM 1),CER3(ECERIFERUM 3) andMAH1(Mid-chain Alkane Hydroxylase 1) in wax biosynthesis and stomatal OCL formation showed that OSP1 may act upstream of CER3 in wax biosynthesis, and implicate that wax composition percentage changes and keeping ketones in a lower level play roles, at least partially, in forming stomatal ledges. Our findings provided insights into the molecular mechanism mediating wax biosynthesis and highlighted the link between wax biosynthesis and the process of stomatal OCL formation. |
| 资助项目 | National Key Research and Development Program of China[2016YFD0100604] ; National Natural Science Foundation[31970730] ; Fundamental Research Funds for the Central Universities[2662017PY034] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000551472600001 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15490]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Hu, Honghong |
| 作者单位 | 1.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 434200, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 3.Huazhong Agr Univ, Coll Life Sci & Technol, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Jing,Yang, Xianpeng,Xiao, Chuanlei,et al. GDSL lipase occluded stomatal pore 1 is required for wax biosynthesis and stomatal cuticular ledge formation[J]. NEW PHYTOLOGIST,2020:17. |
| APA | Tang, Jing.,Yang, Xianpeng.,Xiao, Chuanlei.,Li, Jiaying.,Chen, Yongqiang.,...&Hu, Honghong.(2020).GDSL lipase occluded stomatal pore 1 is required for wax biosynthesis and stomatal cuticular ledge formation.NEW PHYTOLOGIST,17. |
| MLA | Tang, Jing,et al."GDSL lipase occluded stomatal pore 1 is required for wax biosynthesis and stomatal cuticular ledge formation".NEW PHYTOLOGIST (2020):17. |
入库方式： OAI收割
来源：武汉植物园
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